ArticleMolecular biology of the cell2011
A novel organelle, the piNG-body, in the nuage of Drosophila male germ cells is associated with piRNA-mediated gene silencing.
Article in Molecular biology of the cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 50 citations in OpenAlex.
- Tudor domain containing protein 5-like identifies a novel germline body and regulates maternal RNAs during oogenesis in Drosophila.Genetics · 2025Article
- FG repeats drive co-clustering of nuclear pores and P granules in the C. elegans germline.Development (Cambridge, England) · 2025Article
- Non-gonadal expression of piRNAs is widespread across Arthropoda.FEBS letters · 2025Review
- Essential functions of RNA helicase Vasa in maintaining germline stem cells and piRNA-guidedFrontiers in cell and developmental biology · 2024Article
- A maternally programmed intergenerational mechanism enables male offspring to make piRNAs from Y-linked precursor RNAs in Drosophila.Nature cell biology · 2023Article
- RNA Helicase Vasa as a Multifunctional Conservative Regulator of Gametogenesis in Eukaryotes.Current issues in molecular biology · 2023Review
- Review
- The Tudor Domain-Containing Protein, Kotsubu (CG9925), Localizes to the Nuage and Functions in piRNA Biogenesis inFrontiers in molecular biosciences · 2022Article
- Nuage condensates: accelerators or circuit breakers for sRNA silencing pathways?RNA (New York, N.Y.) · 2022Review
- Rhino breaks the deadlock in Drosophila testis.PLoS genetics · 2021Article
- RDC complex executes a dynamic piRNA program during Drosophila spermatogenesis to safeguard male fertility.PLoS genetics · 2021Article
- Dynamic sex chromosome expression in Drosophila male germ cells.Nature communications · 2021Article
- Transcriptome analysis implicates secondary metabolite production, redox reactions, and programmed cell death during allorecognition in Cryphonectria parasitica.G3 (Bethesda, Md.) · 2021Article
- An Emerging Role for Post-translational Modifications in Regulating RNP Condensates in the Germ Line.Frontiers in molecular biosciences · 2021Review
- Phase Separation in Germ Cells and Development.Developmental cell · 2020Review
- A kinesin Klp10A mediates cell cycle-dependent shuttling of Piwi between nucleus and nuage.PLoS genetics · 2020Article
- Review
- Functional Significance of Satellite DNAs: Insights FromFrontiers in cell and developmental biology · 2020Review
- Assembly and Function of Gonad-Specific Non-Membranous Organelles inNon-coding RNA · 2019Review
- piRNA silencing contributes to interspecies hybrid sterility and reproductive isolation in Drosophila melanogaster.Nucleic acids research · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proteins of the PIWI subfamily Aub and AGO3 associated with the germline-specific perinuclear granules (nuage) are involved in the silencing of retrotransposons and other selfish repetitive elements in the Drosophila genome. PIWI proteins and their 25- to 30-nt PIWI-interacting RNA (piRNAs) are considered as key participants of the piRNA pathway. Using immunostaining, we found a large, nuage-associated organelle in the testes, the piNG-body (piRNA nuage giant body), which was significantly more massive than an ordinary nuage granule. This body contains known ovarian nuage proteins, including Vasa, Aub, AGO3, Tud, Spn-E, Bel, Squ, and Cuff, as well as AGO1, the key component of the microRNA pathway. piNG-bodies emerge at the primary spermatocyte stage of spermatogenesis during the period of active transcription. Aub, Vasa, and Tud are located at the periphery of the piNG-body, whereas AGO3 is found in its core. Mutational analysis revealed that Vasa, Aub, and AGO3 were crucial for both the maintenance of the piNG-body structure and the silencing of selfish Stellate repeats. The piNG-body destruction caused by csul mutations that abolish specific posttranslational symmetrical arginine methylation of PIWI proteins is accompanied by strong derepression of Stellate genes known to be silenced via the piRNA pathway.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.