ArticleThe Journal of general virology2011
North American West Nile virus genotype isolates demonstrate differential replicative capacities in response to temperature.
Article in The Journal of general virology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it, 33 citations in OpenAlex.
- Predictive modeling of West Nile virus transmission risk in the Mediterranean Basin: how far from landing?International journal of environmental research and public health · 2013Pooled it
- Rising temperatures contribute to West Nile virus diversification and increased transmission potential.Scientific reports · 2025Article
- Epidemiology and Transmission Dynamics of Viral Encephalitides in West Africa.Infectious disease reports · 2023Review
- Evaluation of NS4A, NS4B, NS5 and 3'UTR Genetic Determinants of WNV Lineage 1 Virulence in Birds and Mammals.Viruses · 2023Article
- A panel of real-time PCR assays for the detection of Bourbon virus, Heartland virus, West Nile virus, andJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2021Article
- Molecular Determinants of West Nile Virus Virulence and Pathogenesis in Vertebrate and Invertebrate Hosts.International journal of molecular sciences · 2020Review
- N-linked glycosylation of the West Nile virus envelope protein is not a requisite for avian virulence or vector competence.PLoS neglected tropical diseases · 2019Article
- Increases in the competitive fitness of West Nile virus isolates after introduction into California.Virology · 2018Article
- Rise and fall of vector infectivity during sequential strain displacements by mosquito-borne dengue virus.Journal of evolutionary biology · 2016Article
- Plasticity of a critical antigenic determinant in the West Nile virus NY99 envelope protein domain III.Virology · 2016Article
- West Nile Virus Temperature Sensitivity and Avian Virulence Are Modulated by NS1-2B Polymorphisms.PLoS neglected tropical diseases · 2016Article
- Climate change projections of West Nile virus infections in Europe: implications for blood safety practices.Environmental health : a global access science source · 2016Article
- Article
- Extrinsic Incubation Rate is Not Accelerated in Recent California Strains of West Nile Virus in Culex tarsalis (Diptera: Culicidae).Journal of medical entomology · 2015Article
- Climate change impacts on West Nile virus transmission in a global context.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Review
- Comparing competitive fitness of West Nile virus strains in avian and mosquito hosts.PloS one · 2015Article
- In vitro study of the replication capacity of the RGNNV and the SJNNV betanodavirus genotypes and their natural reassortants in response to temperature.Veterinary research · 2014Article
- Vector competence of Culex pipiens quinquefasciatus (Diptera: Culicidae) for West Nile virus isolates from Florida.Tropical medicine & international health : TM & IH · 2014Article
- Host competence and helicase activity differences exhibited by West Nile viral variants expressing NS3-249 amino acid polymorphisms.PloS one · 2014Article
- Allele-specific qRT-PCR demonstrates superior detection of single nucleotide polymorphisms as genetic markers for West Nile virus compared to Luminex® and quantitative sequencing.Journal of virological methods · 2014Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The presence of West Nile virus (WNV) was first documented in California, USA, during the summer of 2003, and subsequently the virus has become endemic throughout the state. Sequence analysis has demonstrated that the circulating strains are representative of the North American (WN02) genotype that has displaced the East Coast genotype (NY99). A recent study has indicated that enhanced vector competence at elevated temperatures may have played a role in the displacement of the East Coast genotype by WN02. In the current study, four WN02 strains from California, including an initial 2003 isolate (COAV997), were compared to strain NY99 in growth curve assays in mosquito and duck embryonic fibroblast (DEF) cell lines at differing, biologically relevant temperatures to assess the relative temperature sensitivities of these natural isolates. COAV997 was significantly debilitated in viral replication in DEF cells at 44 °C. Full-length sequence comparison of COAV997 against the NY99 reference strain revealed non-synonymous mutations in the envelope glycoprotein (V159A), non-structural protein 1 (NS1) (K110N) and non-structural protein 4A (NS4A) (F92L), as well as two mutations in the 3' UTR: C→T at nt 10 772 and A→G at nt 10 851. These non-synonymous mutations were introduced into the NY99 viral backbone by site-directed mutagenesis. A mutant containing the NS1-K110N and NS4A-F92L mutations exhibited a debilitated growth phenotype in DEF cells at 44 °C, similar to that of COAV997. One explanation for the subsistence of this genotype is that COAV997 was obtained from an area of California where avian host species might not present elevated temperatures. These data indicate that the NS1 and NS4A mutations identified in some WN02 isolates could reduce thermal stability and impede replication of virus at temperatures observed in febrile avian hosts.
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