ArticleJournal of clinical microbiology2011
Evaluation and verification of the Seeplex Diarrhea-V ACE assay for simultaneous detection of adenovirus, rotavirus, and norovirus genogroups I and II in clinical stool specimens.
Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 59 citations in OpenAlex.
- PCR-Based Versus Conventional Stool Testing in Hospitalized Patients with Diarrhea: Diagnostic Yield, Clinical Impact, and Stewardship Implications.Microorganisms · 2025Article
- Clinical Impact of Stool Polymerase Chain Reaction (PCR) Testing in Hospitalized Patients with Acute Diarrhea: A Retrospective Observational Study.Biomedicines · 2025Article
- Anti-influenza A virus activity of two Newtonia species and the isolated compound myricetin-3-o-rhamnoside.BMC complementary medicine and therapies · 2021Article
- The application research of xTAG GPP multiplex PCR in the diagnosis of persistent and chronic diarrhea in children.BMC pediatrics · 2020Article
- Validation of a Laboratory-Developed Triplex Molecular Assay for Simultaneous Detection of Gastrointestinal Adenovirus and Rotavirus in Stool Specimens.Pathogens (Basel, Switzerland) · 2020Article
- Outcomes and clinical relevance of stool multiplex bacterial polymerase chain reaction in patients with acute diarrhea: single center experience.The Korean journal of internal medicine · 2020Article
- An Overview of the Molecular Methods in the Diagnosis of Gastrointestinal Infectious Diseases.International journal of microbiology · 2020Review
- Evaluation of the performance of a multiplex reverse transcription polymerase chain reaction kit as a potential diagnostic and surveillance kit for rotavirus in Kenya.Tropical diseases, travel medicine and vaccines · 2019Article
- Evaluation of a New Multiplex Real-Time PCR Assay for Detecting Gastroenteritis-Causing Viruses in Stool Samples.Annals of laboratory medicine · 2018Article
- Epidemiology and Factors Related to Clinical Severity of Acute Gastroenteritis in Hospitalized Children after the Introduction of Rotavirus Vaccination.Journal of Korean medical science · 2017Article
- Recent viral pathogen in acute gastroenteritis: a retrospective study at a tertiary hospital for 1 year.Korean journal of pediatrics · 2016Article
- Molecular Diagnostic Methods for Detection and Characterization of Human Noroviruses.The open microbiology journal · 2016Article
- Clinical Significance of Fecal Lactoferrin and Multiplex Polymerase Chain Reaction in Patients with Acute Diarrhea.Gut and liver · 2015Article
- Development and Evaluation of a Loop-Mediated Isothermal Amplification Assay for the Detection of Adenovirus 40 and 41.Food and environmental virology · 2015Article
- Identification of Bufavirus-1 and Bufavirus-3 in Feces of Patients with Acute Diarrhea, China.Scientific reports · 2015Article
- Acute diarrhea in adults consulting a general practitioner in France during winter: incidence, clinical characteristics, management and risk factors.BMC infectious diseases · 2014Article
- A current overview of commercially available nucleic acid diagnostics approaches to detect and identify human gastroenteritis pathogens.Biomolecular detection and quantification · 2014Review
- Evaluation of an immunochromatographic assay for the rapid and simultaneous detection of rotavirus and adenovirus in stool samples.Annals of laboratory medicine · 2014Article
- Simultaneous detection of viral and bacterial enteric pathogens using the Seeplex® Diarrhea ACE detection system.Epidemiology and infection · 2013Article
- Acute viral gastroenteritis in children hospitalized in Iksan, Korea during December 2010-June 2011.Korean journal of pediatrics · 2013Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acute viral gastroenteritis is an intestinal infection that can be caused by several different viruses. Here we describe the evaluation and verification of Seeplex Diarrhea-V ACE (Seeplex DV), a novel commercial multiplex reverse transcription-PCR (RT-PCR) assay that detects 5 diarrheal pathogens, including adenovirus, rotavirus, norovirus genogroup I (GI) and GII, and astrovirus. We describe a retrospective study of 200 clinical specimens of which 177 were stool specimens previously tested for the presence of gastrointestinal viruses by electron microscopy (EM) and/or real-time RT-PCR (rRT-PCR). The remaining 23 specimens comprised other human pathogens of viral or bacterial origin. Discordant norovirus GI and GII results were resolved using a commercial kit; discordant adenovirus and rotavirus results were resolved using a home brew multiplex rRT-PCR assay. Diagnostic sensitivities and specificities were calculated before and after discordant analysis. After discordant analysis, estimated diagnostic sensitivities were 100% for adenovirus, rotavirus, and norovirus GI and 97% for norovirus GII. Diagnostic specificities after discordant analysis were 100% for adenovirus, rotavirus, and norovirus GI and 99.4% for norovirus GII. The 95% limits of detection were 31, 10, 2, and 1 genome equivalent per reaction for adenovirus, rotavirus, and norovirus GI and GII, respectively. The results demonstrate that the Seeplex DV assay is sensitive, specific, convenient, and reliable for the simultaneous detection of several viral pathogens directly in specimens from patients with gastroenteritis. Importantly, this novel multiplex PCR assay enabled the identification of viral coinfections in 12 (6.8%) stool specimens.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.