Evidence map›Paper›PMID 21775543›Full record

ArticleJournal of clinical microbiology2011

A novel eukaryote-made thermostable DNA polymerase which is free from bacterial DNA contamination.

Hideki Niimi, Masashi Mori, Homare Tabata, Hiroshi Minami, Tomohiro Ueno, Shirou Hayashi, Isao Kitajima

Open access · greenAbstract read
In one paragraph

Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
0.9field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 40 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Hideki NiimiClinical Laboratory Center, Toyama University Hospital, 2630 Sugitani Toyama 930-0194, Japan. hiniimi@med.u-toyama.ac.jp
Masashi Mori
Homare Tabata
Hiroshi Minami
Tomohiro Ueno
Shirou Hayashi
Isao Kitajima
Toyama University Hospital · JPMitsui Chemicals (Japan) · JPIshikawa Prefectural University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

To achieve the production of a thermostable DNA polymerase free from bacterial DNA contamination, we developed eukaryote-made thermostable DNA (Taq) polymerase. The novel eukaryote-made thermostable DNA polymerase resolves the problem of contaminating bacterial DNA in conventional bacterially made thermostable DNA polymerase as a result of its manufacture and incomplete purification. Using eukaryote-made thermostable DNA polymerase, the sensitive and reliable detection of bacteria becomes feasible for large fields, thereby making the development of a wide range of powerful applications possible.

Indexed as

BiotechnologyDNA, BacterialRecombinant ProteinsSaccharomyces cerevisiaeTaq PolymeraseDNA, BacterialRecombinant ProteinsTaq Polymerase

Identifiers

PMID21775543
PMCPMC3165589
OpenAlexW2162006416

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.