ArticleJournal of clinical microbiology2011
Multiplex 5' nuclease-quantitative PCR for diagnosis of relapsing fever in a large Tanzanian cohort.
Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 2 syntheses or guidelines pooled it, 16 citations in OpenAlex.
- Neglected etiologies of prolonged febrile illnesses in tropical and subtropical regions: A systematic review.PLoS neglected tropical diseases · 2024Pooled it
- Tick borne relapsing fever - a systematic review and analysis of the literature.PLoS neglected tropical diseases · 2022Pooled it
- Characterization of Immunological Responses toMicrobiology spectrum · 2022Article
- A Comparative Study of Body Lice and Bed Bugs Reveals Factors Potentially Involved in Differential Vector Competence for the Relapsing Fever Spirochete Borrelia recurrentis.Infection and immunity · 2022Article
- Simultaneous Detection and Differentiation of Clinically Relevant Relapsing FeverJournal of clinical microbiology · 2021Article
- Blood Smears Have Poor Sensitivity for Confirming Borrelia miyamotoi Disease.Journal of clinical microbiology · 2019Article
- Dynamics of Spirochetemia and Early PCR Detection of Borrelia miyamotoi.Emerging infectious diseases · 2018Article
- Development and Clinical Validation of a Multiplex Real-Time Quantitative PCR Assay for Human Infection by Anaplasma phagocytophilum and Ehrlichia chaffeensis.Tropical medicine and infectious disease · 2018Article
- Improved sensitivity for molecular detection of bacterial and Candida infections in blood.Journal of clinical microbiology · 2014Article
- Borrelia crocidurae infection in acutely febrile patients, Senegal.Emerging infectious diseases · 2014Article
- Short-term malaria reduction by single-dose azithromycin during mass drug administration for trachoma, Tanzania.Emerging infectious diseases · 2014Article
- Common epidemiology of Rickettsia felis infection and malaria, Africa.Emerging infectious diseases · 2013Article
- Relapsing fever borreliae in Africa.The American journal of tropical medicine and hygiene · 2013Review
- Multiplex real-time PCR diagnostic of relapsing fevers in Africa.PLoS neglected tropical diseases · 2013Article
- Detection of Borrelia persica Infection in Ornithodoros tholozani Using PCR Targeting rrs Gene and Xenodiagnosis.Iranian journal of public health · 2011Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
Abstract
Relapsing fever (RF) is caused by tick- and louse-borne Borrelia spp., is characterized by recurrent fever, and is often misdiagnosed as malaria. Because of submicroscopic bacteremia, microscopy can be insensitive between febrile bouts. We designed a multiplex quantitative PCR (qPCR) assay to distinguish RF Borrelia from Plasmodium falciparum and P. vivax. The assay specifically (100%) amplified pathogenic RF Borrelia (1 copy/reaction). We then tested blood from participants within a Tanzanian cohort assessed at scheduled intervals and with fever. Among 8,617 blood samples from 2,057 participants surveyed routinely, 7 (0.08%) samples and 7 (0.3%) participants had RF DNA (median, 4.4 × 10(3) copies/ml). Of 382 samples from 310 febrile persons, 15 (3.9%) samples from 13 (4.2%) participants had RF DNA (median, 7.9 × 10(2) copies/ml). Five (1.3%) samples from 4 (1.3%) participants were found to harbor Borrelia by microscopy. We conclude that multiplex qPCR holds promise for improved clinical diagnosis and epidemiologic assessment of RF.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.