ArticleJournal of clinical microbiology2011
Carbapenemase activity detection by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 113 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
113 citing papers in PubMed, 1 synthesis or guideline pooled it, 303 citations in OpenAlex.
- Accuracy of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of clinical pathogenic fungi: a meta-analysis.Journal of clinical microbiology · 2014Pooled it
- Evaluation of three variants of the carbapenem inactivation method for carbapenemase detection inJAC-antimicrobial resistance · 2026Article
- Combining mass spectrometry and machine learning models for predicting Klebsiella pneumoniae antimicrobial resistance: a multicenter experience from clinical isolates in Italy.BMC microbiology · 2026Article
- High-Resolution Melting Curve Analysis (HRMA) for the Identification of Class D β-Lactamases (CHDLs) inInfection and drug resistance · 2026Article
- Nanomaterials for bacterial enrichment and detection in healthcare.Nanomedicine (London, England) · 2025Review
- Parallel Detection of the Unamplified Carbapenem Resistance GenesBiosensors · 2025Article
- Application of matrix-assisted laser desorption/ionization time-of flight mass spectrometry in clinical testing and diagnosis.Frontiers in cellular and infection microbiology · 2025Review
- A comparison of two MALDI-TOF MS based assays for the detection of carbapenemases in Enterobacterales.Scientific reports · 2024Article
- Rapid detection of ceftriaxone-resistant Salmonella by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry combined with the ratio of optical density.Annals of clinical microbiology and antimicrobials · 2024Article
- MAST® D72C test: a novel option for ESBL, AmpC and carbapenemase detection.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2024Article
- [DISTRIBUTION OF CARBAPENEMASES IN TUNISIAN BURN PATIENTS].Annals of burns and fire disasters · 2024Article
- A Current Diagnostic and Therapeutic Challenge: Tinea Capitis.Journal of clinical medicine · 2024Article
- Article
- Recent Advances in Colorimetric Tests for the Detection of Infectious Diseases and Antimicrobial Resistance.Diagnostics (Basel, Switzerland) · 2023Review
- MALDI-TOF MS Indirect Beta-Lactamase Detection in Ampicillin-ResistantMicroorganisms · 2023Article
- Preferred β-lactone synthesis can explain high rate of false-negative results in the detection of OXA-48-like carbapenemases.Scientific reports · 2022Article
- Clinical microbiology in detection and identification of emerging microbial pathogens: past, present and future.Emerging microbes & infections · 2022Article
- An Overview on Phenotypic and Genotypic Characterisation of Carbapenem-ResistantMedicina (Kaunas, Lithuania) · 2022Review
- "Omic" Approaches to Bacteria and Antibiotic Resistance Identification.International journal of molecular sciences · 2022Review
- Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry with Time-of-Flight Peak Analysis for Rapid and Accurate Detection of Group B Streptococcus in Pregnant Women.Microbiology spectrum · 2022Article
53 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry is used for the determination of molecular weights of different chemical compounds. We describe here the use of MALDI-TOF mass spectrometry to detect a carbapenem antibiotic, meropenem, and its degradation products. Buffered meropenem solution (0.1 mM Tris-HCl, pH 6.8) was mixed with an overnight culture of bacteria. After 3-h incubation, the reaction mixture was centrifuged, and the supernatant was analyzed by MALDI-TOF mass spectrometry. The presence or absence of peaks representing meropenem and its sodium salts was crucial. The average turnaround time of this test, considering the use of overnight culture, is 4 h. We validated this method for the detection of resistance to carbapenems in Enterobacteriaceae and Pseudomonas aeruginosa mediated by carbapenemase production. A total of 124 strains, including 30 carbapenemase-producing strains, were used in the study. The sensitivity of this method is 96.67%, with a specificity of 97.87%. Our results demonstrate the ability of this method to routinely detect carbapenemases in Enterobacteriaceae and Pseudomonas spp. in laboratories. This assay is comparable with a labor-intensive imipenem-hydrolyzing spectrophotometric assay that is a reference method for the detection of carbapenemase. As demonstrated here, MALDI-TOF mass spectrometry may be used in microbiological laboratories not only for microbial identification but also for other applications, such as studies of mechanisms of antibiotic resistance.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.