ArticleAmerican journal of physiology. Renal physiology2011
Acute laminar shear stress reversibly increases human glomerular endothelial cell permeability via activation of endothelial nitric oxide synthase.
Article in American journal of physiology. Renal physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 32 citations in OpenAlex.
- Fenestrated Endothelial Cells across Organs: Insights into Kidney Function and Disease.International journal of molecular sciences · 2024Review
- Repurposing Riociguat to Target a Novel Paracrine Nitric Oxide-TRPC6 Pathway to Prevent Podocyte Injury.International journal of molecular sciences · 2021Article
- TRIM27 contributes to glomerular endothelial cell injury in lupus nephritis by mediating the FoxO1 signaling pathway.Laboratory investigation; a journal of technical methods and pathology · 2021Article
- A 3-dimensional microfluidic platform for modeling human extravillous trophoblast invasion and toxicological screening.Lab on a chip · 2021Article
- Laminar flow substantially affects the morphology and functional phenotype of glomerular endothelial cells.PloS one · 2021Article
- Mechanical forces and metabolic changes cooperate to drive cellular memory and endothelial phenotypes.Current topics in membranes · 2021Review
- Adaptation of endothelial cells to shear stress under atheroprone conditions by modulating internalization of vascular endothelial cadherin and vinculin.Annals of translational medicine · 2020Article
- Endothelial Dysfunction in Chronic Kidney Disease, from Biology to Clinical Outcomes: A 2020 Update.Journal of clinical medicine · 2020Review
- Shear stress is normalized in glomerular capillaries following ⅚ nephrectomy.American journal of physiology. Renal physiology · 2015Article
- The effect of oscillatory mechanical stimulation on osteoblast attachment and proliferation.Materials science & engineering. C, Materials for biological applications · 2015Article
- Sox18 preserves the pulmonary endothelial barrier under conditions of increased shear stress.Journal of cellular physiology · 2014Article
- Impairment of autophagy in endothelial cells prevents shear-stress-induced increases in nitric oxide bioavailability.Canadian journal of physiology and pharmacology · 2014Article
- Prevalence of Restless Leg Syndrome, Associated Variables and Impact on Sleep Quality in a Cohort of Patients with Neuropsychiatric Disorders.Nigerian medical journal : journal of the Nigeria Medical AssociationArticle
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Laminar shear stress is a key determinant of systemic vascular behavior, including through activation of endothelial nitric oxide synthase (eNOS), but little is known of its role in the glomerulus. We confirmed eNOS expression by glomerular endothelial cells (GEnC) in tissue sections and examined effects of acute exposure (up to 24 h) to physiologically relevant levels of laminar shear stress (10-20 dyn/cm(2)) in conditionally immortalized human GEnC. Laminar shear stress caused an orientation of GEnC and stress fibers parallel to the direction of flow and induced Akt and eNOS phosphorylation along with NO production. Inhibition of the phophatidylinositol (PI)3-kinase/Akt pathway attenuated laminar shear stress-induced eNOS phosphorylation and NO production. Laminar shear stress of 10 dyn/cm(2) had a dramatic effect on GEnC permeability, reversibly decreasing the electrical resistance across GEnC monolayers. Finally, the laminar shear stress-induced reduction in electrical resistance was attenuated by the NOS inhibitors l-N(G)-monomethyl arginine (l-NMMA) and l-N(G)-nitroarginine methyl ester (l-NAME) and also by inhibition of the PI3-kinase/Akt pathway. Hence we have shown for GEnC in vitro that acute permeability responses to laminar shear stress are dependent on NO, produced via activation of the PI3-kinase/Akt pathway and increased eNOS phosphorylation. These results suggest the importance of laminar shear stress and NO in regulating the contribution of GEnC to the permeability properties of the glomerular capillary wall.
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