ArticleJournal of virology2011
An externalized polypeptide partitions between two distinct sites on genome-released poliovirus particles.
Article in Journal of virology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 43 citations in OpenAlex.
- Open questions in enterovirus uncoating.Journal of virology · 2026Review
- Mutation of the highly conserved amino acids in the N-terminal region of the EV-A71 VP1 protein impairs viral RNA release during virus entry.Microbiology spectrum · 2026Article
- Review
- Neutralizing human monoclonal antibodies to poliovirus map to the receptor binding site.Nature communications · 2026Article
- Genomic characterization of novel bat kobuviruses in Madagascar: Implications for viral evolution and zoonotic risk.PloS one · 2025Article
- Cryo-EM structures reveal two distinct conformational states in a picornavirus cell entry intermediate.PLoS pathogens · 2020Article
- Structures of Coxsackievirus A10 unveil the molecular mechanisms of receptor binding and viral uncoating.Nature communications · 2018Article
- Antibody-induced uncoating of human rhinovirus B14.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Beta-Propiolactone Inactivation of Coxsackievirus A16 Induces Structural Alteration and Surface Modification of Viral Capsids.Journal of virology · 2017Article
- Structure of Aichi Virus 1 and Its Empty Particle: Clues to Kobuvirus Genome Release Mechanism.Journal of virology · 2016Article
- Virion Structure of Israeli Acute Bee Paralysis Virus.Journal of virology · 2016Article
- Structure and Genome Release Mechanism of the Human Cardiovirus Saffold Virus 3.Journal of virology · 2016Article
- The novel asymmetric entry intermediate of a picornavirus captured with nanodiscs.Science advances · 2016Article
- Raman spectroscopic signatures of echovirus 1 uncoating.Journal of virology · 2014Article
- The Rhinovirus subviral a-particle exposes 3'-terminal sequences of its genomic RNA.Journal of virology · 2014Article
- Kinetic and structural analysis of coxsackievirus B3 receptor interactions and formation of the A-particle.Journal of virology · 2014Article
- Human enterovirus 71 uncoating captured at atomic resolution.Journal of virology · 2014Article
- Cryo-electron microscopy reconstruction shows poliovirus 135S particles poised for membrane interaction and RNA release.Journal of virology · 2014Article
- Protection against lethal enterovirus 71 challenge in mice by a recombinant vaccine candidate containing a broadly cross-neutralizing epitope within the VP2 EF loop.Theranostics · 2014Article
- Uncoating of common cold virus is preceded by RNA switching as determined by X-ray and cryo-EM analyses of the subviral A-particle.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 1 country.
Funding
Abstract
During cell entry, native poliovirus (160S) converts to a cell-entry intermediate (135S) particle, resulting in the externalization of capsid proteins VP4 and the amino terminus of VP1 (residues 1 to 53). Externalization of these entities is followed by release of the RNA genome (uncoating), leaving an empty (80S) particle. The antigen-binding fragment (Fab) of a monospecific peptide 1 (P1) antibody, which was raised against a peptide corresponding to amino-terminal residues 24 to 40 of VP1, was utilized to track the location of the amino terminus of VP1 in the 135S and 80S states of poliovirus particles via cryogenic electron microscopy (cryo-EM) and three-dimensional image reconstruction. On 135S, P1 Fabs bind to a prominent feature on the external surface known as the "propeller tip." In contrast, our initial 80S-P1 reconstruction showed P1 Fabs also binding to a second site, at least 50 Å distant, at the icosahedral 2-fold axes. Further analysis showed that the overall population of 80S-P1 particles consisted of three kinds of capsids: those with P1 Fabs bound only at the propeller tips, P1 Fabs bound only at the 2-fold axes, or P1 Fabs simultaneously bound at both positions. Our results indicate that, in 80S particles, a significant fraction of VP1 can deviate from icosahedral symmetry. Hence, this portion of VP1 does not change conformation synchronously when switching from the 135S state. These conclusions are compatible with previous observations of multiple conformations of the 80S state and suggest that movement of the amino terminus of VP1 has a role in uncoating. Similar deviations from icosahedral symmetry may be biologically significant during other viral transitions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.