ArticleThe Journal of biological chemistry2011
Effects of simulated microgravity on expression profile of microRNA in human lymphoblastoid cells.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Changes in mitochondrial homeostasis and redox status in astronauts following long stays in space.Scientific reports · 2016Trial
- Impact of modeled microgravity stress on innate immunity in a beneficial animal-microbe symbiosis.Scientific reports · 2024Article
- Omics Studies of Tumor Cells under Microgravity Conditions.International journal of molecular sciences · 2024Review
- Simulating Space Conditions Evokes Different DNA Damage Responses in Immature and Mature Cells of the Human Hematopoietic System.International journal of molecular sciences · 2023Article
- Evaluation of the Effects of Microgravity on Activated Primary Human Hepatic Stellate Cells.International journal of molecular sciences · 2022Article
- Post-Transcriptional Dynamics is Involved in Rapid Adaptation to Hypergravity in Jurkat T Cells.Frontiers in cell and developmental biology · 2022Article
- Detrimental effects of simulated microgravity on mast cell homeostasis and function.Frontiers in immunology · 2022Article
- A dry immersion model of microgravity modulates platelet phenotype, miRNA signature, and circulating plasma protein biomarker profile.Scientific reports · 2021Article
- Genomic Changes Driven by Radiation-Induced DNA Damage and Microgravity in Human Cells.International journal of molecular sciences · 2021Review
- Rapid Transient Transcriptional Adaptation to Hypergravity in Jurkat T Cells Revealed by Comparative Analysis of Microarray and RNA-Seq Data.International journal of molecular sciences · 2021Article
- Metabolic Dynamics in Short- and Long-Term Microgravity in Human Primary Macrophages.International journal of molecular sciences · 2021Article
- Integrative Analysis of Regulatory Module Reveals Associations of Microgravity with Dysfunctions of Multi-body Systems and Tumorigenesis.International journal of molecular sciences · 2020Article
- The Role of C-X-C Chemokine Receptor Type 4 (CXCR4) in Cell Adherence and Spheroid Formation of Human Ewing's Sarcoma Cells under Simulated Microgravity.International journal of molecular sciences · 2019Article
- Simulated microgravity with floating environment promotes migration of non-small cell lung cancers.Scientific reports · 2019Article
- Physiological Responses of Jurkat Lymphocytes to Simulated Microgravity Conditions.International journal of molecular sciences · 2019Article
- Rapid coupling between gravitational forces and the transcriptome in human myelomonocytic U937 cells.Scientific reports · 2018Article
- A microRNA signature and TGF-β1 response were identified as the key master regulators for spaceflight response.PloS one · 2018Article
- Dynamic gene expression response to altered gravity in human T cells.Scientific reports · 2017Article
- Upregulation of miR-223 in the rat liver inhibits proliferation of hepatocytes under simulated microgravity.Experimental & molecular medicine · 2017Article
- Transcriptomics, NF-κB Pathway, and Their Potential Spaceflight-Related Health Consequences.International journal of molecular sciences · 2017Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study explores the changes in expression of microRNA (miRNA) and related genes under simulated microgravity conditions. In comparison with static 1 × g, microgravity has been shown to alter global gene expression patterns and protein levels in cultured cells or animals. miRNA has recently emerged as an important regulator of gene expression, possibly regulating as many as one-third of all human genes. However, very little is known about the effect of altered gravity on miRNA expression. To test the hypothesis that the miRNA expression profile would be altered in zero gravity resulting in altered regulation of gene expression leading to metabolic or functional changes in cells, we cultured TK6 human lymphoblastoid cells in a high aspect ratio vessel (bioreactor) for 72 h either in the rotating condition to model microgravity in space or in the static condition as a control. Expression of several miRNAs was changed significantly in the simulated microgravity condition including miR-150, miR-34a, miR-423-5p, miR-22, miR-141, miR-618, and miR-222. To confirm whether this altered miRNA expression correlates with gene expression and functional changes of the cells, we performed DNA microarray and validated the related genes using quantitative RT-PCR. Expression of several transcription factors including EGR2, ETS1, and c-REL was altered in simulated microgravity conditions. Taken together, the results reported here indicate that simulated microgravity alters the expression of miRNAs and genes in TK6 cells. To our knowledge, this study is the first to report the effects of simulated microgravity on the expression of miRNA and related genes.
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