ArticleBiomaterials2011
Engineered polymer-media interfaces for the long-term self-renewal of human embryonic stem cells.
Article in Biomaterials, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed.
- Primed human pluripotent stem cell-derived blastocyst-like cell aggregates with partial lineage specification.Regenerative therapy · 2025Article
- A Cross-Linked Cyclosiloxane Polymer Matrix as a Platform Enabling Long-Term Culture of Human Induced Pluripotent Stem Cells with Naïve-Like Features.Biomaterials research · 2025Article
- Advancements in theFrontiers in toxicology · 2025Review
- Deciphering the dynamic single-cell transcriptional landscape in the ocular surface ectoderm differentiation system.Life medicine · 2024Article
- Enhanced Expansion of Human Pluripotent Stem Cells and Somatic Cell Reprogramming Using Defined and Xeno-Free Culture Conditions.Bioengineering (Basel, Switzerland) · 2023Article
- A chemically-defined plastic scaffold for the xeno-free production of human pluripotent stem cells.Scientific reports · 2022Article
- Co-delivery of phagocytosis checkpoint and STING agonist by a Trojan horse nanocapsule for orthotopic glioma immunotherapy.Theranostics · 2022Article
- Micro/nanoengineered technologies for human pluripotent stem cells maintenance and differentiation.Nano today · 2021Article
- Discovery of a Novel Polymer for Xeno-Free, Long-Term Culture of Human Pluripotent Stem Cell Expansion.Advanced healthcare materials · 2021Article
- Review
- Expansion Culture of Human Pluripotent Stem Cells and Production of Cardiomyocytes.Bioengineering (Basel, Switzerland) · 2019Review
- Xeno-free culture of human pluripotent stem cells on oligopeptide-grafted hydrogels with various molecular designs.Scientific reports · 2017Article
- Identification of polymer surface adsorbed proteins implicated in pluripotent human embryonic stem cell expansion.Biomaterials science · 2016Article
- Fibroblast-Derived Extracellular Matrix Induces Chondrogenic Differentiation in Human Adipose-Derived Mesenchymal Stromal/Stem Cells in Vitro.International journal of molecular sciences · 2016Article
- Biomaterials for pluripotent stem cell engineering: From fate determination to vascularization.Journal of materials chemistry. B · 2016Article
- Pluripotent stem cell derived hepatocytes: using materials to define cellular differentiation and tissue engineering.Journal of materials chemistry. B · 2016Review
- Engineered Human Stem Cell Microenvironments.Current stem cell reports · 2016Article
- Biological Effects of Culture Substrates on Human Pluripotent Stem Cells.Stem cells international · 2016Review
- Long-term xeno-free culture of human pluripotent stem cells on hydrogels with optimal elasticity.Scientific reports · 2015Article
- Wnt/β-Catenin and MEK-ERK Signaling are Required for Fibroblast-Derived Extracellular Matrix-Mediated Endoderm Differentiation of Embryonic Stem Cells.Stem cell reviews and reports · 2015Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
We have developed a synthetic polymer interface for the long-term self-renewal of human embryonic stem cells (hESCs) in defined media. We successfully cultured hESCs on hydrogel interfaces of aminopropylmethacrylamide (APMAAm) for over 20 passages in chemically-defined mTeSR™1 media and demonstrated pluripotency of multiple hESC lines with immunostaining and quantitative RT-PCR studies. Results for hESC proliferation and pluripotency markers were both qualitatively and quantitatively similar to cells cultured on Matrigel™-coated substrates. Mechanistically, it was resolved that bovine serum albumin (BSA) in the mTeSR™1 media was critical for cell adhesion on APMAAm hydrogel interfaces. This study uniquely identified a robust long-term culture surface for the self-renewal of hESCs without the use of biologic coatings (e.g., peptides, proteins, or Matrigel™) in completely chemically-defined media that employed practical culturing techniques amenable to clinical-scale cell expansion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.