ArticleBiomaterials2011
Intracellular trafficking pathways involved in the gene transfer of nano-structured calcium phosphate-DNA particles.
Article in Biomaterials, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The trial behind it
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Who cites it
13 citing papers in PubMed, 50 citations in OpenAlex.
- Article
- Review
- Improved CaP Nanoparticles for Nucleic Acid and Protein Delivery to Neural Primary Cultures and Stem Cells.ACS nano · 2024Article
- Strategy for Cytoplasmic Delivery Using Inorganic Particles.Pharmaceutical research · 2022Review
- Biodegradable calcium phosphate nanoparticles for cancer therapy.Advances in colloid and interface science · 2020Review
- In vitro and in vivo mechanism of hepatocellular carcinoma inhibition by β-TCP nanoparticles.International journal of nanomedicine · 2019Article
- Hydroxyapatite as a Vehicle for the Selective Effect of Superparamagnetic Iron Oxide Nanoparticles against Human Glioblastoma Cells.ACS applied materials & interfaces · 2017Article
- Amiloride-enhanced gene transfection of octa-arginine functionalized calcium phosphate nanoparticles.PloS one · 2017Article
- Gene delivery using calcium phosphate nanoparticles: Optimization of the transfection process and the effects of citrate and poly(l-lysine) as additives.Journal of colloid and interface science · 2016Article
- Calcium Phosphate as a Key Material for Socially Responsible Tissue Engineering.Materials (Basel, Switzerland) · 2016Article
- A sight on the current nanoparticle-based gene delivery vectors.Nanoscale research letters · 2014Article
- Pegylated siRNA-loaded calcium phosphate nanoparticle-driven amplification of cancer cell internalization in vivo.Biomaterials · 2013Article
- Delivery system for DNAzymes using arginine-modified hydroxyapatite nanoparticles for therapeutic application in a nasopharyngeal carcinoma model.International journal of nanomedicine · 2013Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Nano-structured calcium phosphate (NanoCaP) particles have been proven to be a powerful means of non-viral gene delivery. In order to better understand the mechanisms through which NanoCaPs-mediated mammalian cell transfection is achieved, we have sought to define the intracellular trafficking pathways involved in the cellular uptake and intracellular processing of these particles. Previous work has indicated that NanoCaP-DNA complexes are most likely internalized via endocytosis, however the subsequent pathways involved have not been determined. Through the use of specific inhibitors, we show that endocytosis of NanoCaP particles is both clathrin- and caveolae-dependent, and suggest that the caveolaer mechanism is the major contributor. We demonstrate colocalization of NanoCaP-pDNA complexes with known markers of both clathrin-coated and caveolar vesicles. Furthermore, through the use of quantitative flow cytometry, we present the first work in which the percent internalization of CaP-DNA complexes into cells is quantified. The overall goal of this research is to foster the continued improvement of NanoCaP-based gene delivery strategies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.