Evidence map›Paper›PMID 21773933›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2011

Quantification of asymmetric microtubule nucleation at subcellular structures.

Xiaodong Zhu, Irina Kaverina

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Detection of Microtubule Nucleation Hotspots at the Golgi.Methods in molecular biology (Clifton, N.J.) · 2020
    Article
  5. Golgi as an MTOC: making microtubules for its own good.Histochemistry and cell biology · 2013
    Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Xiaodong ZhuDepartment of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN, USA. xiaodong.zhu@vanderbilt.edu
Irina Kaverina

Funding

Spatial Regulation of Cytoskeletal AsymmetryR01GM078373 · NIGMS · VANDERBILT UNIVERSITY · PI KAVERINA, IRINA · 2008 to 2017
$3.2M
NIGMS NIH HHS R01 GM078373
6 · The paper itself

Abstract

Cell polarization is important for multiple physiological processes. In polarized cells, microtubules (MTs) are organized into a spatially polarized array. Generally, in nondifferentiated cells, it is assumed that MTs are symmetrically nucleated exclusively from centrosome [microtubule organizing center (MTOC)] and then reorganized into the asymmetric array. We have recently identified the Golgi complex as an additional MTOC that asymmetrically nucleates MTs toward one side of the cell. Methods used for alternative MTOC identification include microtubule regrowth after complete drug-induced depolymerization and tracking of growing microtubules using fluorescently labeled MT +TIP binding proteins in living cells. These approaches can be used for quantification of MT nucleation sites at diverse subcellular structures.

Indexed as

AnimalsCentrosomeFluorescent Antibody TechniqueGolgi ApparatusHumansMicrotubule-Organizing CenterMicrotubules

Identifiers

PMID21773933
PMCPMC3282175

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.