ArticleJournal of fluorescence2011
Determination of copper(II) ion concentration by lifetime measurements of green fluorescent protein.
Article in Journal of fluorescence, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Copper stress upregulates oxidative stress response, histidine production, and iron acquisition genes inFEMS microbes · 2026Article
- Fluorescent Protein-Based Sensors for Detecting Essential Metal Ions across the Tree of Life.ACS sensors · 2024Review
- Genetically encoded fluorescent sensors for metals in biology.Current opinion in chemical biology · 2023Review
- Mutant Flavin-Based Fluorescent Protein Sensors for Detecting Intracellular Zinc and Copper inACS sensors · 2022Article
- Transition Metals Induce Quenching of Monomeric Near-Infrared Fluorescent Proteins.Biochemistry · 2022Article
- Monitoring Transmembrane and Peripheral Membrane Protein Interactions by Förster Resonance Energy Transfer Using Fluorescence Lifetime Imaging Microscopy.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Fluorescence depolarization dynamics of ionic strength sensors using time-resolved anisotropy.Biophysical journal · 2021Article
- Fluorescence of a Histidine-Modified Enhanced Green Fluorescent Protein (EGFP) Effectively Quenched by Copper(II) Ions. Part II. Molecular Determinants.Journal of fluorescence · 2015Article
- Fluorescence lifetime excitation cytometry by kinetic dithering.Electrophoresis · 2014Article
- Time-resolved photoluminescence spectroscopy and imaging: new approaches to the analysis of cultural heritage and its degradation.Sensors (Basel, Switzerland) · 2014Article
- Fluorescence of a histidine-modified enhanced green fluorescent protein (EGFP) effectively quenched by copper(II) ions.Journal of fluorescence · 2013Article
- Highly selective and sensitive chemosensor for Hg2+ based on the naphthalimide fluorophore.Journal of fluorescence · 2012Article
- Investigation of copper homeostasis in plant cells by fluorescence lifetime imaging microscopy.Plant signaling & behavior · 2012Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The understanding of cellular processes and functions and the elucidation of their physiological mechanisms is an important aim in the life sciences. One important aspect is the uptake and the release of essential substances as well as their interactions with the cellular environment. As green fluorescent protein (GFP) can be genetically encoded in cells it can be used as an internal sensor giving a deeper insight into biochemical pathways. Here we report that the presence of copper(II) ions leads to a decrease of the fluorescence lifetime (τ(fl)) of GFP and provide evidence for Förster resonance energy transfer (FRET) as the responsible quenching mechanism. We identify the His(6)-tag as the responsible binding site for Cu(2+) with a dissociation constant K(d) = 9 ± 2 μM and a Förster radius R(0) = 2.1 ± 0.1 nm. The extent of the lifetime quenching depends on [Cu(2+)] which is comprehended by a mathematical titration model. We envision that Cu(2+) can be quantified noninvasively and in real-time by measuring τ(fl) of GFP.
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Registered trials
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