ArticleFrontiers in microbiology2010
Host Kinase Activity is Required for Coxiella burnetii Parasitophorous Vacuole Formation.
Article in Frontiers in microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
- To die or not to die: Programmed cell death responses and their interactions with Coxiella burnetii infection.Molecular microbiology · 2022Article
- Neurotransmitter System-Targeting Drugs Antagonize Growth of the Q Fever Agent, Coxiella burnetii, in Human Cells.mSphere · 2021Article
- Involvement ofFrontiers in cellular and infection microbiology · 2021Review
- Chlamydia trachomatis recruits protein kinase C during infection.Pathogens and disease · 2019Article
- Identification and characterization of arginine finger-like motifs, and endosome-lysosome basolateral sorting signals within the Coxiella burnetii type IV secreted effector protein CirA.Microbes and infection · 2018Article
- The Type IV Secretion System Effector Protein CirA Stimulates the GTPase Activity of RhoA and Is Required for Virulence in a Mouse Model of Coxiella burnetii Infection.Infection and immunity · 2016Article
- Article
- Contrasting Lifestyles Within the Host Cell.Microbiology spectrum · 2016Review
- Coxiella burnetii Phagocytosis Is Regulated by GTPases of the Rho Family and the RhoA Effectors mDia1 and ROCK.PloS one · 2015Article
- Identification of novel Coxiella burnetii Icm/Dot effectors and genetic analysis of their involvement in modulating a mitogen-activated protein kinase pathway.Infection and immunity · 2014Article
- Coxiella burnetii exploits host cAMP-dependent protein kinase signalling to promote macrophage survival.Cellular microbiology · 2014Article
- Identification of Coxiella burnetii type IV secretion substrates required for intracellular replication and Coxiella-containing vacuole formation.Journal of bacteriology · 2013Article
- Molecular pathogenesis of the obligate intracellular bacterium Coxiella burnetii.Nature reviews. Microbiology · 2013Review
- Virulent Coxiella burnetii pathotypes productively infect primary human alveolar macrophages.Cellular microbiology · 2013Article
- Coxiella burnetii alters cyclic AMP-dependent protein kinase signaling during growth in macrophages.Infection and immunity · 2012Article
- The obligate intracellular lifestyle.Frontiers in microbiology · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Coxiella burnetii is the etiologic agent of human Q fever and targets alveolar phagocytic cells in vivo wherein the pathogen generates a phagolysosome-like parasitophorous vacuole (PV) for replication. C. burnetii displays a prolonged growth cycle, making PV maintenance critical for bacterial survival. Previous studies showed that C. burnetii mediates activation of eukaryotic kinases to inhibit cell death, indicating the importance of host signaling during infection. In the current study, we examined the role of eukaryotic kinase signaling in PV establishment. A panel of 113 inhibitors was analyzed for their impact on C. burnetii infection of human THP-1 macrophage-like cells and HeLa cells. Inhibition of 11 kinases or two phosphatases altered PV formation and prevented pathogen growth, with most inhibitor-treated cells harboring organisms in tight-fitting phagosomes, indicating kinase/phosphatase activation is required for PV maturation. Five inhibitors targeted protein kinase C (PKC), suggesting a critical role for this protein during intracellular growth. The PKC-specific substrate MARCKS was phosphorylated at 24 h post-infection and remained phosphorylated through 5 days post-infection, indicating prolonged regulation of the PKC pathway by C. burnetii. Infection also altered the activation status of p38, myosin light chain kinase, and cAMP-dependent protein kinase, suggesting C. burnetii subverts numerous phosphorylation cascades. These results underscore the importance of intracellular host signaling for C. burnetii PV biogenesis.
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