ArticleMolecular systems biology2011
Absolute quantification of microbial proteomes at different states by directed mass spectrometry.
Article in Molecular systems biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 104 citations in OpenAlex.
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- Core-sheath coupling controls flagellar curvature and motility inbioRxiv : the preprint server for biology · 2026Article
- Imaging of plant calcium-sensor kinase conformation monitors real time calcium-dependent decoding in planta.The Plant cell · 2024Article
- Histones with an unconventional DNA-binding mode in vitro are major chromatin constituents in the bacterium Bdellovibrio bacteriovorus.Nature microbiology · 2023Article
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- Molecular response to PARP1 inhibition in ovarian cancer cells as determined by mass spectrometry based proteomics.Journal of ovarian research · 2021Article
- Single Cell Proteomics by Data-Independent Acquisition To Study Embryonic Asymmetry inAnalytical chemistry · 2019Article
- Multifaceted Stoichiometry Control of Bacterial Operons Revealed by Deep Proteome Quantification.Frontiers in genetics · 2019Article
- Application of targeted mass spectrometry in bottom-up proteomics for systems biology research.Journal of proteomics · 2018Review
- Elusive data underlying debate at the prokaryote-eukaryote divide.Biology direct · 2018Article
- The binding landscape of a partially-selective isopeptidase inhibitor with potent pro-death activity, based on the bis(arylidene)cyclohexanone scaffold.Cell death & disease · 2018Article
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- Pathogenic Leptospires Modulate Protein Expression and Post-translational Modifications in Response to Mammalian Host Signals.Frontiers in cellular and infection microbiology · 2017Article
- Discovery Proteomics Identifies a Molecular Link between the Coatomer Protein Complex I and Androgen Receptor-dependent Transcription.The Journal of biological chemistry · 2016Article
- Analysis of LexA binding sites and transcriptomics in response to genotoxic stress in Leptospira interrogans.Nucleic acids research · 2016Article
- The quantitative and condition-dependent Escherichia coli proteome.Nature biotechnology · 2016Article
- Comprehensive proteomics analysis of glycosomes from Leishmania donovani.Omics : a journal of integrative biology · 2015Article
- Quantitative proteomic analysis reveals a simple strategy of global resource allocation in bacteria.Molecular systems biology · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 5 institutions in 4 countries.
Funding
Abstract
Over the past decade, liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) has evolved into the main proteome discovery technology. Up to several thousand proteins can now be reliably identified from a sample and the relative abundance of the identified proteins can be determined across samples. However, the remeasurement of substantially similar proteomes, for example those generated by perturbation experiments in systems biology, at high reproducibility and throughput remains challenging. Here, we apply a directed MS strategy to detect and quantify sets of pre-determined peptides in tryptic digests of cells of the human pathogen Leptospira interrogans at 25 different states. We show that in a single LC-MS/MS experiment around 5000 peptides, covering 1680 L. interrogans proteins, can be consistently detected and their absolute expression levels estimated, revealing new insights about the proteome changes involved in pathogenic progression and antibiotic defense of L. interrogans. This is the first study that describes the absolute quantitative behavior of any proteome over multiple states, and represents the most comprehensive proteome abundance pattern comparison for any organism to date.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.