ArticleVirology journal2011
Infectious alphavirus production from a simple plasmid transfection+.
Article in Virology journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed.
- A High-Throughput Antiviral Assay Based on a Sindbis Virus-GFP for the Discovery of Inhibitors of Alphavirus Replication.Viruses · 2026Article
- An in vitro workflow to create and modify infectious clones using replication cycle reaction.Virology · 2023Article
- Construction of a Chikungunya Virus, Replicon, and Helper Plasmids for Transfection of Mammalian Cells.Viruses · 2022Article
- Electrochemical Capillary Driven Immunoassay for Detection of SARS-CoV-2.ACS measurement science au · 2022Article
- Understanding Molecular Pathogenesis with Chikungunya Virus Research Tools.Current topics in microbiology and immunology · 2022Review
- Sindbis Virus Replication Reduces Dependence on Mitochondrial Metabolism During Infection.Frontiers in cellular and infection microbiology · 2022Article
- Rolling circle amplification: A high fidelity and efficient alternative to plasmid preparation for the rescue of infectious clones.Virology · 2020Article
- Single-Round Infectious Particle Production by DNA-Launched Infectious Clones of Bungowannah Pestivirus.Viruses · 2020Article
- Identification of small molecule inhibitors of the Chikungunya virus nsP1 RNA capping enzyme.Antiviral research · 2018Article
- Dissecting the Role of E2 Protein Domains in Alphavirus Pathogenicity.Journal of virology · 2015Article
- A novel system for visualizing alphavirus assembly.Journal of virological methods · 2015Article
- Article
- Identifying the Role of E2 Domains on Alphavirus Neutralization and Protective Immune Responses.PLoS neglected tropical diseases · 2015Article
- Novel vaccine against Venezuelan equine encephalitis combines advantages of DNA immunization and a live attenuated vaccine.Vaccine · 2013Article
- Article
- Identification of a novel antiviral inhibitor of the flavivirus guanylyltransferase enzyme.Journal of virology · 2012Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
We have developed a new method for producing infectious double subgenomic alphaviruses from plasmids transfected into mammalian cells. A double subgenomic Sindbis virus (TE3'2J) was transcribed from a cytomegalovirus PolII promoter, which results in the production of infectious virus. Transfection of as little as 125 ng of plasmid is able to produce 1 × 10(8) plaque forming units/ml (PFU/ml) of infectious virus 48 hours post-transfection. This system represents a more efficient method for producing recombinant Sindbis viruses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.