ArticlePlant physiology2011
Propidium iodide competes with Ca(2+) to label pectin in pollen tubes and Arabidopsis root hairs.
Article in Plant physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
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Who cites it
69 citing papers in PubMed, 152 citations in OpenAlex.
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- Synthetic pectin-cellulose nanofiber capsule recapitulates the mechanical properties of a regenerating plant cell wall.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Papaver S-determinants trigger mitochondrially derived ROS production and disrupt energy metabolism in incompatible pollen tubes.The Plant cell · 2026Article
- The epidermis coordinates multi-scale symmetry breaking in chiral root growth.Nature communications · 2025Article
- Mechanosensitive ion channel MSL8 is required for oscillatory growth and cell wall dynamics in Arabidopsis pollen tubes.Plant reproduction · 2025Article
- Triphenylphosphonium is an effective targeting moiety for plant mitochondria.The New phytologist · 2025Article
- Effect of UV-B stress on olive (Olea europaea L.) pollen tubes: A study of callose plug deposition and male germ unit integrity.Protoplasma · 2025Article
- Hygroscopic movements of cone scale of white firFrontiers in plant science · 2025Article
- Optimizing Visualization of Pollen Tubes in Wheat Pistils.Plants (Basel, Switzerland) · 2024Article
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- Spearmint targets microtubules by (-)-carvone.Horticulture research · 2024Article
- Aromatic amino acid biosynthesis impacts root hair development and symbiotic associations in Lotus japonicus.Plant physiology · 2023Article
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- Application of Green-enhanced Nano-lantern as a bioluminescent ratiometric indicator for measurement of Arabidopsis thaliana root apoplastic fluid pH.Plant, cell & environment · 2022Article
- GhBZR3 suppresses cotton fiber elongation by inhibiting very-long-chain fatty acid biosynthesis.The Plant journal : for cell and molecular biology · 2022Article
- Quantification of Species-Preferential Micropylar Chemoattraction inInternational journal of molecular sciences · 2022Article
- Correlated mechanochemical maps ofQuantitative plant biology · 2022Article
9 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have used propidium iodide (PI) to investigate the dynamic properties of the primary cell wall at the apex of Arabidopsis (Arabidopsis thaliana) root hairs and pollen tubes and in lily (Lilium formosanum) pollen tubes. Our results show that in root hairs, as in pollen tubes, oscillatory peaks in PI fluorescence precede growth rate oscillations. Pectin forms the primary component of the cell wall at the tip of both root hairs and pollen tubes. Given the electronic structure of PI, we investigated whether PI binds to pectins in a manner analogous to Ca(2+) binding. We first show that Ca(2+) is able to abrogate PI growth inhibition in a dose-dependent manner. PI fluorescence itself also relies directly on the amount of Ca(2+) in the growth solution. Exogenous pectin methyl esterase treatment of pollen tubes, which demethoxylates pectins, freeing more Ca(2+)-binding sites, leads to a dramatic increase in PI fluorescence. Treatment with pectinase leads to a corresponding decrease in fluorescence. These results are consistent with the hypothesis that PI binds to demethoxylated pectins. Unlike other pectin stains, PI at low yet useful concentration is vital and specifically does not alter the tip-focused Ca(2+) gradient or growth oscillations. These data suggest that pectin secretion at the apex of tip-growing plant cells plays a critical role in regulating growth, and PI represents an excellent tool for examining the role of pectin and of Ca(2+) in tip growth.
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