ArticleThe Journal of biological chemistry2011
Substrate-mediated fidelity mechanism ensures accurate decoding of proline codons.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 26 citations in OpenAlex.
- Mechanistic and evolutionary insights into a family of aminoacyl-tRNA deacylases that protects against canavanine toxicity.Nucleic acids research · 2025Article
- Structural basis of tRNAPro acceptor stem recognition by a bacterial trans-editing domain.Nucleic acids research · 2023Article
- A cysteinyl-tRNA synthetase variant confers resistance against selenite toxicity and decreases selenocysteine misincorporation.The Journal of biological chemistry · 2019Article
- Stoichiometry of triple-sieve tRNA editing complex ensures fidelity of aminoacyl-tRNA formation.Nucleic acids research · 2019Article
- Quality control by trans-editing factor prevents global mistranslation of non-protein amino acid α-aminobutyrate.RNA biology · 2018Article
- Conformational and chemical selection by aProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Stress Response and Adaptation Mediated by Amino Acid Misincorporation during Protein Synthesis.Advances in nutrition (Bethesda, Md.) · 2016Review
- Specificity and catalysis hardwired at the RNA-protein interface in a translational proofreading enzyme.Nature communications · 2015Article
- Distinct tRNA recognition strategies used by a homologous family of editing domains prevent mistranslation.Nucleic acids research · 2014Article
- Strictly conserved lysine of prolyl-tRNA Synthetase editing domain facilitates binding and positioning of misacylated tRNA(Pro.).Biochemistry · 2014Article
- Emergence and evolution.Topics in current chemistry · 2014Review
- Aminoacylation of tRNA 2'- or 3'-hydroxyl by phosphoseryl- and pyrrolysyl-tRNA synthetases.FEBS letters · 2013Article
- Exclusive use of trans-editing domains prevents proline mistranslation.The Journal of biological chemistry · 2013Article
- Aminoacyl-tRNA substrate and enzyme backbone atoms contribute to translational quality control by YbaK.The journal of physical chemistry. B · 2013Article
- Substrate and enzyme functional groups contribute to translational quality control by bacterial prolyl-tRNA synthetase.The journal of physical chemistry. B · 2012Article
- Substrate specificity of bacterial prolyl-tRNA synthetase editing domain is controlled by a tunable hydrophobic pocket.The Journal of biological chemistry · 2012Article
- The α-amino group of the threonine substrate as the general base during tRNA aminoacylation: a new version of substrate-assisted catalysis predicted by hybrid DFT.The journal of physical chemistry. A · 2011Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Aminoacyl-tRNA synthetases attach specific amino acids to cognate tRNAs. Prolyl-tRNA synthetases are known to mischarge tRNA(Pro) with the smaller amino acid alanine and with cysteine, which is the same size as proline. Quality control in proline codon translation is partly ensured by an editing domain (INS) present in most bacterial prolyl-tRNA synthetases that hydrolyzes smaller Ala-tRNA(Pro) and excludes Pro-tRNA(Pro). In contrast, Cys-tRNA(Pro) is cleared by a freestanding INS domain homolog, YbaK. Here, we have investigated the molecular mechanism of catalysis and substrate recognition by Hemophilus influenzae YbaK using site-directed mutagenesis, enzymatic assays of isosteric substrates and functional group analogs, and computational modeling. These studies together with mass spectrometric characterization of the YbaK-catalyzed reaction products support a novel substrate-assisted mechanism of Cys-tRNA(Pro) deacylation that prevents nonspecific Pro-tRNA(Pro) hydrolysis. Collectively, we propose that the INS and YbaK domains co-evolved distinct mechanisms involving steric exclusion and thiol-specific chemistry, respectively, to ensure accurate decoding of proline codons.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.