ArticleThe Journal of biological chemistry2011
SlyA protein activates fimB gene expression and type 1 fimbriation in Escherichia coli K-12.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- Type 1 fimbria and P pili: regulatory mechanisms of the prototypical members of the chaperone-usher fimbrial family.Archives of microbiology · 2024Review
- Regulation ofFrontiers in microbiology · 2022Review
- What Flips the Switch? Signals and Stress Regulating Extraintestinal PathogenicMicroorganisms · 2021Review
- Transcriptome RNA Sequencing Data Set of Differential Gene Expression in Escherichia coli BW25113 Wild-Type andMicrobiology resource announcements · 2021Article
- Strain and host-cell dependent role of type-1 fimbriae in the adherence phenotype of super-shed Escherichia coli O157:H7.International journal of medical microbiology : IJMM · 2021Article
- SlyA Transcriptional Regulator Is Not Directly Affected by ppGpp Levels.Frontiers in microbiology · 2020Article
- SlyA and HilD Counteract H-NS-Mediated Repression on theJournal of bacteriology · 2019Article
- Article
- Article
- Article
- Identification of new members of the Escherichia coli K-12 MG1655 SlyA regulon.Microbiology (Reading, England) · 2017Article
- Molecular Characterization of the Vacuolating Autotransporter Toxin in Uropathogenic Escherichia coli.Journal of bacteriology · 2016Article
- Characterization of SlyA in Shigella flexneri Identifies a Novel Role in Virulence.Infection and immunity · 2016Article
- Article
- Identification and regulation of a novel Citrobacter rodentium gut colonization fimbria (Gcf).Journal of bacteriology · 2015Article
- Article
- RfaH suppresses small RNA MicA inhibition of fimB expression in Escherichia coli K-12.Journal of bacteriology · 2014Article
- OmpR regulation of the uropathogenic Escherichia coli fimB gene in an acidic/high osmolality environment.Microbiology (Reading, England) · 2013Article
- Article
- Regulation ofWorld journal of clinical infectious diseases · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
We have demonstrated that SlyA activates fimB expression and hence type 1 fimbriation, a virulence factor in Escherichia coli. SlyA is shown to bind to two operator sites (O(SA1) and O(SA2)), situated between 194 and 167 base pairs upstream of the fimB transcriptional start site. fimB expression is derepressed in an hns mutant and diminished by a slyA mutation in the presence of H-NS only. H-NS binds to multiple sites in the promoter region, including two sites (H-NS2 and H-NS3) that overlap O(SA1) and O(SA2), respectively. Mutations that disrupt either O(SA1) or O(SA2) eliminate or reduce the activating effect of SlyA but have different effects on the level of expression. We interpret these results as reflecting the relative competition between SlyA and H-NS binding. Moreover we show that SlyA is capable of displacing H-NS from its binding sites in vitro. We suggest SlyA binding prevents H-NS binding to H-NS2 and H-NS3 and the subsequent oligomerization of H-NS necessary for full inhibition of fimB expression. In addition, we show that SlyA activates fimB expression independently of two other known regulators of fimB expression, NanR and NagC. It is demonstrated that the rarely used UUG initiation codon limits slyA expression and that low SlyA levels limit fimB expression. Furthermore, Western blot analysis shows that cells grown in rich-defined medium contain ~1000 SlyA dimers per cell whereas those grown in minimal medium contain >20% more SlyA. This study extends our understanding of the role that SlyA plays in the host-bacterial relationship.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.