ArticleBiophysical journal2011
Probing orientational behavior of MHC class I protein and lipid probes in cell membranes by fluorescence polarization-resolved imaging.
Article in Biophysical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Protein exchange is reduced in calcium-independent epithelial junctions.The Journal of cell biology · 2020Article
- Variable incidence angle linear dichroism (VALiD): a technique for unique 3D orientation measurement of fluorescent ensembles.Optics express · 2020Article
- Desmoglein 3 Order and Dynamics in Desmosomes Determined by Fluorescence Polarization Microscopy.Biophysical journal · 2017Article
- Single molecule optical measurements of orientation and rotations of biological macromolecules.Methods and applications in fluorescence · 2016Article
- Quantitative nanoscale imaging of orientational order in biological filaments by polarized superresolution microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Ultimate use of two-photon fluorescence microscopy to map orientational behavior of fluorophores.Biophysical journal · 2014Article
- The role of molecular dipole orientation in single-molecule fluorescence microscopy and implications for super-resolution imaging.Chemphyschem : a European journal of chemical physics and physical chemistry · 2014Review
- Polarization-controlled TIRFM with focal drift and spatial field intensity correction.Biophysical journal · 2014Article
- Modification of plasma membrane organization in tobacco cells elicited by cryptogein.Plant physiology · 2014Article
- Mapping the local organization of cell membranes using excitation-polarization-resolved confocal fluorescence microscopy.Biophysical journal · 2013Article
- Quantitative scheme for full-field polarization rotating fluorescence microscopy using a liquid crystal variable retarder.The Review of scientific instruments · 2012Article
Corrections and comments
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Authors and funding
7 authors.
Funding
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Abstract
Steady-state polarization-resolved fluorescence imaging is used to analyze the molecular orientational order behavior of rigidly labeled major histocompatibility complex class I (MHC I) proteins and lipid probes in cell membranes of living cells. These fluorescent probes report the orientational properties of proteins and their surrounding lipid environment. We present a statistical study of the molecular orientational order, modeled as the width of the angular distribution of the molecules, for the proteins in the cell endomembrane and plasma membrane, as well as for the lipid probes in the plasma membrane. We apply this methodology on cells after treatments affecting the actin and microtubule networks. We find in particular opposite orientational order changes of proteins and lipid probes in the plasma membrane as a response to the cytoskeleton disruption. This suggests that MHC I orientational order is governed by its interaction with the cytoskeleton, whereas the plasma membrane lipid order is governed by the local cell membrane morphology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.