ArticleIndoor air2011
Assessing allergenic fungi in house dust by floor wipe sampling and quantitative PCR.
Article in Indoor air, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 46 citations in OpenAlex.
- The Indoor Microbiome: Sampling, Analysis and Emerging Trends.Environmental microbiology reports · 2026Pooled it
- A deployable film method to enable replicable sampling of low-abundance environmental microbiomes.Scientific reports · 2024Article
- Distribution of SARS-CoV-2 RNA signal in a home with COVID-19 positive occupants.The Science of the total environment · 2021Article
- Comparison of DNA sequencing and morphological identification techniques to characterize environmental fungal communities.Scientific reports · 2021Article
- The short-term effect of residential home energy retrofits on indoor air quality and microbial exposure: A case-control study.PloS one · 2021Article
- Evaluation of the complexity of indoor air in hospital wards based on PM2.5, real-time PCR, adenosine triphosphate bioluminescence assay, microbial culture and mass spectrometry.BMC infectious diseases · 2019Article
- Storage mite concentrations are underestimated compared to house dust mite concentrations.Experimental & applied acarology · 2019Article
- The developing premature infant gut microbiome is a major factor shaping the microbiome of neonatal intensive care unit rooms.Microbiome · 2018Article
- Exploring temporal patterns of bacterial and fungal DNA accumulation on a ventilation system filter for a Singapore university library.PloS one · 2018Article
- Strain-resolved analysis of hospital rooms and infants reveals overlap between the human and room microbiome.Nature communications · 2017Article
- Protocol Improvements for Low Concentration DNA-Based Bioaerosol Sampling and Analysis.PloS one · 2015Article
- Triazole Susceptibilities in Thermotolerant Fungal Isolates from Outdoor Air in the Seoul Capital Area in South Korea.PloS one · 2015Article
- Beneficial effects of plant-associated microbes on indoor microbiomes and human health?Frontiers in microbiology · 2014Article
- Analysis of the fungal flora in environmental dust samples by PCR-SSCP method.Current microbiology · 2013Article
- Particle-size distributions and seasonal diversity of allergenic and pathogenic fungi in outdoor air.The ISME journal · 2012Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
unlabelledIn the present study, we modified an existing surface wipe sampling method for lead and other heavy metals to create a protocol to collect fungi in floor dust followed by real-time quantitative PCR (qPCR)-based detection. We desired minimal inconvenience for participants in residential indoor environmental quality and health studies. Accuracy, precision, and method detection limits (MDLs) were investigated. Overall, MDLs ranged from 0.6 to 25 cell/cm² on sampled floors. Overall measurement precisions expressed as the coefficient of variation because of sample processing and qPCR ranged 6-63%. Median and maximum fungal concentrations in house dust in study homes in Visalia, Tulare County, California, were 110 and 2500 cell/cm², respectively, with universal fungal primers (allergenic and nonallergenic species). The field study indicated samplings in multiple seasons were necessary to characterize representative whole-year fungal concentrations in residential microenvironments. This was because significant temporal variations were observed within study homes. Combined field and laboratory results suggested this modified new wipe sampling method, in conjunction with growth-independent qPCR, shows potential to improve human exposure and health studies for fungal pathogens and allergens in dust in homes of susceptible, vulnerable population subgroups. PRACTICAL IMPLICATIONS: Fungi are ubiquitous in indoor and outdoor environments, and many fungi are known to cause allergic reactions and exacerbate asthma attacks. This study established--by modifying an existing--a wipe sampling method to collect fungi in floor dust followed by real-time quantitative PCR (qPCR)-based detection methodologies. Results from this combined laboratory and field assessment suggested the methodology's potential to inform larger human exposure studies for fungal pathogens and allergens in house dust as well as epidemiologic studies of children with asthma and older adults with chronic respiratory diseases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.