ArticlePLoS neglected tropical diseases2011
Polyethyleneimine (PEI) mediated siRNA gene silencing in the Schistosoma mansoni snail host, Biomphalaria glabrata.
Article in PLoS neglected tropical diseases, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 52 citations in OpenAlex.
- Snail 3G: genomics, genetics, and gene-editing ofFrontiers in genetics · 2026Review
- CRISPR/Cas9-germline editing ofScience advances · 2025Article
- The chromosome-level genome assembly of the slug Deroceras laeve facilitates its use as a comparative model of regeneration.G3 (Bethesda, Md.) · 2025Article
- Implications of Endogenous Small Regulatory RNAs Survey in Mollusks on Gene Silencing Approaches.Marine biotechnology (New York, N.Y.) · 2025Article
- Inhibition of alternative oxidase disrupts the development and oviposition of Biomphalaria glabrata snails.Parasites & vectors · 2023Article
- Single-cell RNA-seq profiling of individual Biomphalaria glabrata immune cells with a focus on immunologically relevant transcripts.Immunogenetics · 2022Article
- PIWI silencing mechanism involving the retrotransposon nimbus orchestrates resistance to infection with Schistosoma mansoni in the snail vector, Biomphalaria glabrata.PLoS neglected tropical diseases · 2021Article
- Identification and localization of a gonadotropin-releasing hormone-related neuropeptide in Biomphalaria, an intermediate host for schistosomiasis.The Journal of comparative neurology · 2021Article
- Article
- The hemolymph of Biomphalaria snail vectors of schistosomiasis supports a diverse microbiome.Environmental microbiology · 2020Article
- Diminished adherence of Biomphalaria glabrata embryonic cell line to sporocysts of Schistosoma mansoni following programmed knockout of the allograft inflammatory factor.Parasites & vectors · 2020Article
- Sequencing of Transcriptome and Small RNA Revealed the Xenobiotic Metabolism-Related Genes and Potential Regulatory miRNA in Asian Tramp Snail.Frontiers in genetics · 2020Article
- Gene drives for schistosomiasis transmission control.PLoS neglected tropical diseases · 2019Review
- Comparative study of excretory-secretory proteins released by Schistosoma mansoni-resistant, susceptible and naïve Biomphalaria glabrata.Parasites & vectors · 2019Article
- Sequence and structural variation in the genome of the Biomphalaria glabrata embryonic (Bge) cell line.Parasites & vectors · 2018Article
- Bioreducible Poly(Amino Ethers) Based mTOR siRNA Delivery for Lung Cancer.Pharmaceutical research · 2018Article
- Whole genome analysis of a schistosomiasis-transmitting freshwater snail.Nature communications · 2017Article
- Schistosome infectivity in the snail, Biomphalaria glabrata, is partially dependent on the expression of Grctm6, a Guadeloupe Resistance Complex protein.PLoS neglected tropical diseases · 2017Article
- Differential expression of small RNA pathway genes associated with the Biomphalaria glabrata/Schistosoma mansoni interaction.PloS one · 2017Article
- Cytometric analysis, genetic manipulation and antibiotic selection of the snail embryonic cell line Bge from Biomphalaria glabrata, the intermediate host of Schistosoma mansoni.International journal for parasitology · 2015Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
An in vivo, non-invasive technique for gene silencing by RNA interference (RNAi) in the snail, Biomphalaria glabrata, has been developed using cationic polymer polyethyleneimine (PEI) mediated delivery of long double-stranded (ds) and small interfering (si) RNA. Cellular delivery was evaluated and optimized by using a 'mock' fluorescent siRNA. Subsequently, we used the method to suppress expression of Cathepsin B (CathB) with either the corresponding siRNA or dsRNA of this transcript. In addition, the knockdown of peroxiredoxin (Prx) at both RNA and protein levels was achieved with the PEI-mediated soaking method. B. glabrata is an important snail host for the transmission of the parasitic digenean platyhelminth, Schistosoma mansoni that causes schistosomiasis in the neotropics. Progress is being made to realize the genome sequence of the snail and to uncover gene expression profiles and cellular pathways that enable the snail to either prevent or sustain an infection. Using PEI complexes, a convenient soaking method has been developed, enabling functional gene knockdown studies with either dsRNA or siRNA. The protocol developed offers a first whole organism method for host-parasite gene function studies needed to identify key mechanisms required for parasite development in the snail host, which ultimately are needed as points for disrupting this parasite mediated disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.