ArticlePloS one2011
MicroRNA expression and regulation in human ovarian carcinoma cells by luteinizing hormone.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 25 citations in OpenAlex.
- The Mechanisms of BDNF Promoting the Proliferation of Porcine Follicular Granulosa Cells: Role of miR-127 and Involvement of the MAPK-ERK1/2 Pathway.Animals : an open access journal from MDPI · 2023Article
- The Role of Endocrine G Protein-Coupled Receptors in Ovarian Cancer Progression.Frontiers in endocrinology · 2017Review
- Role of microRNA-210 in human intervertebral disc degeneration.Experimental and therapeutic medicine · 2016Article
- miR-761 inhibits tumor progression by targeting MSI1 in ovarian carcinoma.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- Disruptive environmental chemicals and cellular mechanisms that confer resistance to cell death.Carcinogenesis · 2015Review
- HPV16 early gene E5 specifically reduces miRNA-196a in cervical cancer cells.Scientific reports · 2015Article
- Key nodes of a microRNA network associated with the integrated mesenchymal subtype of high-grade serous ovarian cancer.Chinese journal of cancer · 2015Review
- Hsa-miR-301a-3p Acts as an Oncogene in Laryngeal Squamous Cell Carcinoma via Target Regulation of Smad4.Journal of Cancer · 2015Article
- Dietary MicroRNA Database (DMD): An Archive Database and Analytic Tool for Food-Borne microRNAs.PloS one · 2015Article
- MiR-101 suppresses the epithelial-to-mesenchymal transition by targeting ZEB1 and ZEB2 in ovarian carcinoma.Oncology reports · 2014Article
- Altered expression of miRNAs in a dihydrotestosterone-induced rat PCOS model.Journal of ovarian research · 2013Article
- Integrating microRNAs into the complexity of gonadotropin signaling networks.Frontiers in cell and developmental biology · 2013Review
- Alternatively spliced lysyl oxidase-like 4 isoforms have a pro-metastatic role in cancer.Clinical & experimental metastasis · 2013Article
- MicroRNAs synergistically regulate milk fat synthesis in mammary gland epithelial cells of dairy goats.Gene expression · 2013Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundMicroRNAs have been widely-studied with regard to their aberrant expression and high correlation with tumorigenesis and progression in various solid tumors. With the major goal of assessing gonadotropin (luteinizing hormone, LH) contributions to LH receptor (LHR)-positive ovarian cancer cells, we have conducted a genome-wide transcriptomic analysis on human epithelial ovarian cancer cells to identify the microRNA-associated cellular response to LH-mediated activation of LHR.
methodsHuman ovarian cancer cells (SKOV3) were chosen as negative control (LHR-) and stably transfected to express functional LHR (LHR+), followed by incubation with LH (0-20 h). At different times of LH-mediated activation of LHR the cancer cells were analyzed by a high-density Ovarian Cancer Disease-Specific-Array (DSA, ALMAC™), which profiled ∼ 100,000 transcripts with ∼ 400 non-coding microRNAs.
findingsIn total, 65 microRNAs were identified to exhibit differential expression in either LHR expressing SKOV3 cells or LH-treated cells, a few of which have been found in the genomic fragile regions that are associated with abnormal deletion or amplification in cancer, such as miR-21, miR-101-1, miR-210 and miR-301a. By incorporating the dramatic expression changes observed in mRNAs, strong microRNA/mRNA regulatory pairs were predicted through statistical analyses coupled with collective computational prediction. The role of each microRNA was then determined through a functional analysis based on the highly-confident microRNA/mRNA pairs.
conclusionThe overall impact on the transcriptome-level expression indicates that LH may regulate apoptosis and cell growth of LHR+ SKOV3 cells, particularly by reducing cancer cell proliferation, with some microRNAs involved in regulatory roles.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.