ArticleFood and environmental virology2011
Design and Application of Nucleic Acid Standards for Quantitative Detection of Enteric Viruses by Real-Time PCR.
Article in Food and environmental virology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 49 citations in OpenAlex.
- Evaluation and Application of the MIRA-qPCR Method for Rapid Detection of Norovirus Genogroup II in Shellfish.Microorganisms · 2025Article
- Genotyping and Molecular Diagnosis of Hepatitis A Virus in Human Clinical Samples Using Multiplex PCR-Based Next-Generation Sequencing.Microorganisms · 2022Article
- Porcine Blood and Liver as Sporadic Sources of Hepatitis E Virus (HEV) in the Production Chain of Offal-Derived Foodstuffs in Poland.Food and environmental virology · 2021Article
- Hepatitis E Virus in Manure and Its Removal byMicroorganisms · 2020Article
- Whole Genome Sequencing Characterization of HEV3-Microorganisms · 2020Article
- Comparison of targeted next-generation sequencing for whole-genome sequencing of Hantaan orthohantavirus in Apodemus agrarius lung tissues.Scientific reports · 2019Article
- Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017.Frontiers in microbiology · 2019Article
- Assessment of the Virological Quality of Marine and Running Surface Waters in NW Greece: A Case Study.Food and environmental virology · 2018Article
- Presence of Human and Animal Viruses in Surface Waters in Vojvodina Province of Serbia.Food and environmental virology · 2015Article
- Occurrence of Human Cosavirus in Wastewater and River Water in Japan.Food and environmental virology · 2014Article
- Harmonised investigation of the occurrence of human enteric viruses in the leafy green vegetable supply chain in three European countries.Food and environmental virology · 2012Article
- Virus hazards from food, water and other contaminated environments.FEMS microbiology reviews · 2012Review
- Occurrence of human enteric viruses in commercial mussels at retail level in three European countries.Food and environmental virology · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Synthetic multiple-target RNA and DNA oligonucleotides were constructed for use as quantification standards for nucleic acid amplification assays for human norovirus genogroup I and II, hepatitis E virus, murine norovirus, human adenovirus, porcine adenovirus and bovine polyomavirus. This approach overcomes the problems related to the difficulty of obtaining practical quantities of viral RNA and DNA from these viruses. The quantification capacity of assays using the standards was excellent in each case (R(2) > 0.998 and PCR efficiency > 0.89). The copy numbers of the standards were equivalent to the genome equivalents of representative viruses (murine norovirus and human adenovirus), ensuring an accurate determination of virus presence. The availability of these standards should facilitate the implementation of nucleic acid amplification-based methods for quantitative virus detection.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.