ArticleThe Plant cell2011
A novel calcium binding site in the slow vacuolar cation channel TPC1 senses luminal calcium levels.
Article in The Plant cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 97 citations in OpenAlex.
- Review
- Gaining or cutting SLAC: the evolution of plant guard cell signalling pathways.The New phytologist · 2024Article
- Arabidopsis HAK5 under low KNature communications · 2024Article
- Article
- Long-Distance Electrical and Calcium Signals Evoked by Hydrogen Peroxide in Physcomitrella.Plant & cell physiology · 2023Article
- Structure and Function of Plant and Mammalian TPC Channels.Handbook of experimental pharmacology · 2023Article
- TPC1-Type Channels inPlants (Basel, Switzerland) · 2022Article
- Major vacuolar TPC1 channel in stress signaling: what matters, KStress biology · 2022Review
- Molecular basis of multistep voltage activation in plant two-pore channel 1.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Review
- Calcium channels and transporters: Roles in response to biotic and abiotic stresses.Frontiers in plant science · 2022Review
- Crosstalk between Calcium and ROS Signaling during Flg22-Triggered Immune Response inPlants (Basel, Switzerland) · 2021Article
- Voltage-gating and cytosolic CaProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Transport, functions, and interaction of calcium and manganese in plant organellar compartments.Plant physiology · 2021Review
- Computational Analyses of the AtTPC1 (Arabidopsis Two-Pore Channel 1) Permeation Pathway.International journal of molecular sciences · 2021Article
- Deciphering the Role of Ion Channels in Early Defense Signaling against Herbivorous Insects.Cells · 2021Review
- Impact of Mammalian Two-Pore Channel Inhibitors on Long-Distance Electrical Signals in the Characean MacroalgaPlants (Basel, Switzerland) · 2021Article
- Evolutionary Aspects of TRPMLs and TPCs.International journal of molecular sciences · 2020Review
- Salt Stress Signals on Demand: Cellular Events in the Right Context.International journal of molecular sciences · 2020Review
- Resting state structure of the hyperdepolarization activated two-pore channel 3.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytosolic calcium homeostasis is pivotal for intracellular signaling and requires sensing of calcium concentrations in the cytosol and accessible stores. Numerous Ca²⁺ binding sites have been characterized in cytosolic proteins. However, little is known about Ca²⁺ binding inside organelles, like the vacuole. The slow vacuolar (SV) channel, encoded by Arabidopsis thaliana TPC1, is regulated by luminal Ca²⁺. However, the D454/fou2 mutation in TPC1 eliminates vacuolar calcium sensitivity and increases store calcium content. In a search for the luminal calcium binding site, structure modeling indicated a possible coordination site formed by residues Glu-450, Asp-454, Glu-456, and Glu-457 on the luminal side of TPC1. Each Glu residue was replaced by Gln, the modified genes were transiently expressed in loss-of-TPC1-function protoplasts, and SV channel responses to luminal calcium were recorded by patch clamp. SV channels lacking any of the four negatively charged residues appeared altered in calcium sensitivity of channel gating. Our results indicate that Glu-450 and Asp-454 are directly involved in Ca²⁺ binding, whereas Glu-456 and Glu-457 are probably involved in connecting the luminal Ca²⁺ binding site to the channel gate. This novel vacuolar calcium binding site represents a potential tool to address calcium storage in plants.
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