ArticleApplied and environmental microbiology2011
Rapid concentration and molecular enrichment approach for sensitive detection of Escherichia coli and Shigella species in potable water samples.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed.
- Detection and colocalization of STEC genetic markers in wheat flour using whole cell digital droplet PCR.Frontiers in microbiology · 2026Article
- Emerging Bioanalytical Devices and Platforms for Rapid Detection of Pathogens in Environmental Samples.Micromachines · 2022Review
- Comparison of the culture method with multiplex PCR for the confirmation of Legionella spp. and Legionella pneumophila.Journal of applied microbiology · 2021Article
- BCIG-SMAC medium and PMA-qPCR for differential detection of viableIranian journal of microbiology · 2021Article
- Escherichia coli Capacity to Repopulate Microcosms Under Osmotic/U.V. Synergic Stress in Tropical Waters.Current microbiology · 2021Article
- Separation of Escherichia coli from natural samples for identification of sources and microcosm inoculation.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
- A novel method for rapid and sensitive detection of viable Escherichia coli cells using UV-induced PMA-coupled quantitative PCR.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
- Isolation ssDNA aptamers specific for both live and viable but nonculturable stateRSC advances · 2020Article
- The etiology of acute meningitis and encephalitis syndromes in a sentinel pediatric hospital, Shenzhen, China.BMC infectious diseases · 2019Article
- Development of a filtration-based LAMP-LFA method as sensitive and rapid detection ofJournal of food science and technology · 2019Article
- Three-Dimensional Reservoir-Based Dielectrophoresis (rDEP) for Enhanced Particle Enrichment.Micromachines · 2018Article
- Recent developments in detection and enumeration of waterborne bacteria: a retrospective minireview.MicrobiologyOpen · 2016Review
- Survival, Biofilm Formation, and Growth Potential of Environmental and Enteric Escherichia coli Strains in Drinking Water Microcosms.Applied and environmental microbiology · 2016Article
- A comprehensive method for amplicon-based and metagenomic characterization of viruses, bacteria, and eukaryotes in freshwater samples.Microbiome · 2016Article
- Evaluating exposure of pedestrians to airborne contaminants associated with non-potable water use for pavement cleaning.Environmental science and pollution research international · 2016Article
- On-line monitoring of Escherichia coli in raw water at Oset drinking water treatment plant, Oslo (Norway).International journal of environmental research and public health · 2015Article
- Abilities of the mCP Agar method and CRENAME alpha toxin-specific real-time PCR assay to detect Clostridium perfringens spores in drinking water.Applied and environmental microbiology · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
In this work, we used a rapid, simple, and efficient concentration-and-recovery procedure combined with a DNA enrichment method (dubbed CRENAME [concentration and recovery of microbial particles, extraction of nucleic acids, and molecular enrichment]), that we coupled to an Escherichia coli/Shigella-specific real-time PCR (rtPCR) assay targeting the tuf gene, to sensitively detect E. coli/Shigella in water. This integrated method was compared to U.S. Environmental Protection Agency (EPA) culture-based Method 1604 on MI agar in terms of analytical specificity, ubiquity, detection limit, and rapidity. None of the 179 non-E. coli/Shigella strains tested was detected by both methods, with the exception of Escherichia fergusonii, which was detected by the CRENAME procedure combined with the E. coli/Shigella-specific rtPCR assay (CRENAME + E. coli rtPCR). DNA from all 90 E. coli/Shigella strains tested was amplified by the CRENAME + E. coli rtPCR, whereas the MI agar method had limited ubiquity and detected only 65 (72.2%) of the 90 strains tested. In less than 5 h, the CRENAME + E. coli rtPCR method detected 1.8 E. coli/Shigella CFU whereas the MI agar method detected 1.2 CFU/100 ml of water in 24 h (95% confidence). Consequently, the CRENAME method provides an easy and efficient approach to detect as little as one Gram-negative E. coli/Shigella cell present in a 100-ml potable water sample. Coupled with an E. coli/Shigella-specific rtPCR assay, the entire molecular procedure is comparable to U.S. EPA Method 1604 on MI agar in terms of analytical specificity and detection limit but provides significant advantages in terms of speed and ubiquity.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.