ArticleClinical biochemistry2011
Lateral flow assay for simultaneous detection of cellular- and humoral immune responses.
Article in Clinical biochemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed, 1 synthesis or guideline pooled it, 88 citations in OpenAlex.
- Detection of anti-M. leprae antibodies in children in leprosy-endemic areas: A systematic review.PLoS neglected tropical diseases · 2021Pooled it
- The combined effect of chemoprophylaxis with single dose rifampicin and immunoprophylaxis with BCG to prevent leprosy in contacts of newly diagnosed leprosy cases: a cluster randomized controlled trial (MALTALEP study).BMC infectious diseases · 2013Trial
- Inflammatory biomarkers in sebum for identifying skin damage in patients with a Stage I pressure ulcer in the pelvic region: A single centre observational, longitudinal cohort study with elderly patients.International wound journal · 2023Observational
- Engineering Innovative Interfaces for Point-of-Care Diagnostics.Current opinion in colloid & interface science · 2023Review
- Host biomarker-based quantitative rapid tests for detection and treatment monitoring of tuberculosis and COVID-19.iScience · 2023Article
- Lateral Flow Microarray-Based ELISA for Cytokines.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Photoluminescent Molecules and Materials as Diagnostic Reporters in Lateral Flow Assays.ACS applied bio materials · 2022Article
- Quantitative Rapid Test for Detection and Monitoring of Active Pulmonary Tuberculosis in Nonhuman Primates.Biology · 2021Article
- Effect of inhaled anaesthetics gases on cytokines and oxidative stress alterations for the staff health status in hospitals.International archives of occupational and environmental health · 2021Article
- Sensitive and quantitative detection of cardiac troponin I with upconverting nanoparticle lateral flow test with minimized interference.Scientific reports · 2021Article
- Point-of-care detection of cytokines in cytokine storm management and beyond: Significance and challenges.View (Beijing, China) · 2021Review
- Upconverting nanoparticle reporter-based highly sensitive rapid lateral flow immunoassay for hepatitis B virus surface antigen.Analytical and bioanalytical chemistry · 2021Article
- Article
- Double-Antigen Lateral Flow Immunoassay for the Detection of Anti-HIV-1 and -2 Antibodies Using Upconverting Nanoparticle Reporters.Sensors (Basel, Switzerland) · 2021Article
- An anti-BSA antibody-based immunochromatographic assay for chloramphenicol and aflatoxin MMikrochimica acta · 2019Article
- Inorganic Complexes and Metal-Based Nanomaterials for Infectious Disease Diagnostics.Chemical reviews · 2019Review
- Multiplex Lateral Flow Immunoassay: An Overview of Strategies towards High-throughput Point-of-Need Testing.Biosensors · 2018Review
- Evaluation of Immunodiagnostic Tests for Leprosy in Brazil, China and Ethiopia.Scientific reports · 2018Article
- Rapid Multiplexed Detection on Lateral-Flow Devices Using a Laser Direct-Write Technique.Biosensors · 2018Article
- Minimum requirements and optimal testing strategies of a diagnostic test for leprosy as a tool towards zero transmission: A modeling study.PLoS neglected tropical diseases · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
Abstract
objectiveThe development of a cytokine detection assay suitable for detection of multiple biomarkers for improved diagnosis of mycobacterial diseases. DESIGN AND
methodsA lateral flow (LF) assay to detect IL-10 was developed utilizing the up-converting phosphor (UCP) reporter-technology. The assay was evaluated using blood samples of leprosy patients. Multiplex applications were explored targeting: 1) IL-10 and IFN-γ in assay buffer; 2) IL-10 and anti-phenolic glycolipid (PGL-I) antibodies in serum from leprosy patients.
resultsDetection of IL-10 below the targeted level of 100pg/mL in serum was shown. Comparison with ELISA showed a quantitative correlation with R(2) value of 0.92. Multiplexing of cytokines and simultaneous detection of cytokine and antibody was demonstrated.
conclusionsThe UCP-LF IL-10 assay is a user-friendly, rapid alternative for IL-10 ELISAs, suitable for multiplex detection of different cytokines and can be merged with antibody-detection assays to simultaneously detect cellular- and humoral immunity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.