ArticleProteome science2011
Comparative Proteomic Analysis of saccharopolyspora spinosa SP06081 and PR2 strains reveals the differentially expressed proteins correlated with the increase of spinosad yield.
Article in Proteome science, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 30 citations in OpenAlex.
- A High-Throughput Method Based on Microculture Technology for Screening of High-Yield Strains of Tylosin-ProducingJournal of microbiology and biotechnology · 2023Article
- Bacterioferritin: a key iron storage modulator that affects strain growth and butenyl-spinosyn biosynthesis in Saccharopolyspora pogona.Microbial cell factories · 2021Article
- Effects of acuC on the growth development and spinosad biosynthesis of Saccharopolyspora spinosa.Microbial cell factories · 2021Article
- Enhancing the insecticidal activity of new Bacillus thuringiensis X023 by copper ions.Microbial cell factories · 2020Article
- Heterologous expression and antitumor activity analysis of syringolin from Pseudomonas syringae pv. syringae B728a.Microbial cell factories · 2018Article
- High Level of Spinosad Production in the Heterologous Host Saccharopolyspora erythraea.Applied and environmental microbiology · 2016Article
- Proteomic analysis of the influence of Cu(2+) on the crystal protein production of Bacillus thuringiensis X022.Microbial cell factories · 2015Article
- Metabolic engineering of rational screened Saccharopolyspora spinosa for the enhancement of spinosyns A and D production.Molecules and cells · 2014Article
- Differential proteomic profiling reveals regulatory proteins and novel links between primary metabolism and spinosad production in Saccharopolyspora spinosa.Microbial cell factories · 2014Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSaccharopolyspora spinosa produces the environment-friendly biopesticide spinosad, a mixture of two polyketide-derived macrolide active ingredients called spinosyns A and D. Therefore considerable interest is in the improvement of spinosad production because of its low yield in wild-type S. spinosa. Recently, a spinosad-hyperproducing PR2 strain with stable heredity was obtained from protoplast regeneration of the wild-type S. spinosa SP06081 strain. A comparative proteomic analysis was performed on the two strains during the first rapid growth phase (RG1) in seed medium (SM) by using label-free quantitative proteomics to investigate the underlying mechanism leading to the enhancement of spinosad yield.
resultsIn total, 224 proteins from the SP06081 strain and 204 proteins from the PR2 strain were unambiguously identified by liquid chromatography-tandem mass spectrometry analysis, sharing 140 proteins. A total of 12 proteins directly related to spinosad biosynthesis were identified from the two strains in RG1. Comparative analysis of the shared proteins revealed that approximately 31% of them changed their abundance significantly and fell in all of the functional groups, such as tricarboxylic acid cycles, glycolysis, biosynthetic processes, catabolic processes, transcription, translation, oxidation and reduction. Several key enzymes involved in the synthesis of primary metabolic intermediates used as precursors for spinosad production, energy supply, polyketide chain assembly, deoxysugar methylation, and antioxidative stress were differentially expressed in the same pattern of facilitating spinosad production by the PR2 strain. Real-time reverse transcriptase polymerase chain reaction analysis revealed that four of five selected genes showed a positive correlation between changes at the translational and transcriptional expression level, which further confirmed the proteomic analysis.
conclusionsThe present study is the first comprehensive and comparative proteome analysis of S. spinosa strains. Our results highlight the differentially expressed proteins between the two S. spinosa strains and provide some clues to understand the molecular and metabolic mechanisms that could lead to the increased spinosad production yield.
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