ArticleBioconjugate chemistry2011
Intracellular trafficking of polyamidoamine-poly(ethylene glycol) block copolymers in DNA delivery.
Article in Bioconjugate chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
14 citing papers in PubMed.
- Cell uptake and intracellular trafficking of bioreducible poly(amidoamine) nanoparticles for efficient mRNA translation in chondrocytes.Frontiers in bioengineering and biotechnology · 2023Article
- Stochastic simulations of nanoparticle internalization through transferrin receptor dependent clathrin-mediated endocytosis.Biochimica et biophysica acta. General subjects · 2018Article
- Photo-induced Leishmania DNA degradation by silver-doped zinc oxide nanoparticle: an in-vitro approach.IET nanobiotechnology · 2016Article
- Flow shear stress differentially regulates endothelial uptake of nanocarriers targeted to distinct epitopes of PECAM-1.Journal of controlled release : official journal of the Controlled Release Society · 2015Article
- Controlled Endolysosomal Release of Agents by pH-responsive Polymer Blend Particles.Pharmaceutical research · 2015Article
- A plug-and-play ratiometric pH-sensing nanoprobe for high-throughput investigation of endosomal escape.Biomaterials · 2015Article
- Getting across the cell membrane: an overview for small molecules, peptides, and proteins.Methods in molecular biology (Clifton, N.J.) · 2015Review
- Reducible chimeric polypeptide consisting of octa-D-arginine and tetra-L-histidine peptides as an efficient gene delivery vector.International journal of nanomedicine · 2015Article
- Article
- FRET imaging reveals different cellular entry routes of self-assembled and disulfide bonded polymeric micelles.Molecular pharmaceutics · 2013Article
- Article
- Crosslinked linear polyethylenimine enhances delivery of DNA to the cytoplasm.Journal of controlled release : official journal of the Controlled Release Society · 2013Article
- Enhanced bioactivity of internally functionalized cationic dendrimers with PEG cores.Biomacromolecules · 2012Article
- Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
The delivery of nucleic acids has the potential to revolutionize medicine by allowing previously untreatable diseases to be clinically addressed. Viral delivery systems have shown immunogenicity and toxicity dangers, but synthetic vectors have lagged in transfection efficiency. Previously, we developed a modular, linear-dendritic block copolymer architecture with high gene transfection efficiency compared to commercial standards. This rationally designed system makes use of a cationic dendritic block to condense the anionic DNA and forms complexes with favorable endosomal escape properties. The linear block provides biocompatibility and protection from serum proteins, and can be functionalized with a targeting ligand. In this work, we quantitate performance of this system with respect to intracellular barriers to gene delivery using both high-throughput and traditional approaches. An image-based, high-throughput assay for endosomal escape is described and applied to the block copolymer system. Nuclear entry is demonstrated to be the most significant barrier to more efficient delivery and will be addressed in future versions of the system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.