ArticlePloS one2011
Lentiviral mediated transgenesis by in vivo manipulation of spermatogonial stem cells.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 29 citations in OpenAlex.
- 14-3-3ε conditional knockout mice exhibit defects in the development of the epidermis.FEBS letters · 2024Article
- Viral Transduction of Mammalian Spermatogonial Stem Cells.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Testis electroporation coupled with autophagy inhibitor to treat non-obstructive azoospermia.Molecular therapy. Nucleic acids · 2022Article
- The human EF1a promoter does not provide expression of the transgene in mice.Transgenic research · 2022Article
- Interaction between alpha-COP and SMN ameliorates disease phenotype in a mouse model of spinal muscular atrophy.Biochemical and biophysical research communications · 2019Article
- Infectivity of adeno-associated virus serotypes in mouse testis.BMC biotechnology · 2018Article
- Intratesticular injection followed by electroporation allows gene transfer in caprine spermatogenic cells.Scientific reports · 2018Article
- Gene delivery to Nile tilapia cells for transgenesis and the role of PI3K-c2α in angiogenesis.Scientific reports · 2017Article
- Nucleic acids delivery methods for genome editing in zygotes and embryos: the old, the new, and the old-new.Biology direct · 2016Review
- An efficient strategy for generation of transgenic mice by lentiviral transduction of male germline stem cells in vivo.Journal of animal science and biotechnology · 2015Article
- MMP7 is required to mediate cell invasion and tumor formation upon Plakophilin3 loss.PloS one · 2015Article
- Rescuing infertility of Pick1 knockout mice by generating testis-specific transgenic mice via testicular infection.Scientific reports · 2013Article
- Direct modification of spermatogonial stem cells using lentivirus vectorsAsian journal of andrologyArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This report describes a technique for the generation of transgenic mice by in vivo manipulation of spermatogonial stem cells with a high rate of success. Spermatogonial stem cells (SSCs) in pre-pubescent animals were infected in vivo with recombinant lentiviruses expressing EGFP-f and mated with normal females. All male pre-founder mice produced transgenic pups with an overall success rate of over 60%. The transgene was heritable and the pre-founder mice could be used in multiple mating experiments. This technology could be used to perform overexpression/knockdown screens in vivo using bar-coded lentiviruses, thus permitting the design of genetic screens in the mouse. Further, this technology could be adapted to other laboratory animals resulting in the generation of model systems that closely approximate human development and disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.