ArticlePloS one2011
β-1,3-Glucan-induced host phospholipase D activation is involved in Aspergillus fumigatus internalization into type II human pneumocyte A549 cells.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 64 citations in OpenAlex.
- Systemic Aspergillosis in Dogs: A Historical and Current State-of-the-Art Review.Veterinary sciences · 2026Review
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- Epithelial uptake leads to fungal killingiScience · 2024Article
- Accelerating the understanding ofCurrent research in microbial sciences · 2024Review
- Pathogenicity and virulence ofVirulence · 2023Review
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- Immunity to fungi in the lung.Seminars in immunology · 2023Review
- Novel Insights intoJournal of fungi (Basel, Switzerland) · 2022Review
- Pathogenesis of Respiratory Viral and Fungal Coinfections.Clinical microbiology reviews · 2022Review
- Complement receptor 3 mediatesVirulence · 2021Article
- Application of laser capture microdissection and PCR sequencing in the diagnosis ofEmerging microbes & infections · 2021Review
- Aspergillus fumigatus Induces the Release of IL-8 and MCP-1 by Activating Nuclear Transcription Through Dectin-1 and CR3 Receptors in Alveolar Epithelial Cells.Current microbiology · 2021Article
- Characterisation ofJournal of fungi (Basel, Switzerland) · 2021Article
- Mechanisms of fungal dissemination.Cellular and molecular life sciences : CMLS · 2021Review
- Fungal dysbiosis of the gut microbiota is associated with colorectal cancer in Chinese patients.American journal of translational research · 2021Article
- Variation of virulence of five Aspergillus fumigatus isolates in four different infection models.PloS one · 2021Article
- PC945, a Novel Inhaled Antifungal Agent, for the Treatment of Respiratory Fungal Infections.Journal of fungi (Basel, Switzerland) · 2020Review
- Influences of the Culturing Media in the Virulence and Cell Wall ofJournal of fungi (Basel, Switzerland) · 2020Article
- Aspergillus fumigatus Cell Wall Promotes Apical Airway Epithelial Recruitment of Human Neutrophils.Infection and immunity · 2020Article
- EphA2-Dependent Internalization ofFrontiers in microbiology · 2020Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The internalization of Aspergillus fumigatus into lung epithelial cells is a process that depends on host cell actin dynamics. The host membrane phosphatidylcholine cleavage driven by phospholipase D (PLD) is closely related to cellular actin dynamics. However, little is known about the impact of PLD on A. fumigatus internalization into lung epithelial cells. Here, we report that once germinated, A. fumigatus conidia were able to stimulate host PLD activity and internalize more efficiently in A549 cells without altering PLD expression. The internalization of A. fumigatus in A549 cells was suppressed by the downregulation of host cell PLD using chemical inhibitors or siRNA interference. The heat-killed swollen conidia, but not the resting conidia, were able to activate host PLD. Further, β-1,3-glucan, the core component of the conidial cell wall, stimulated host PLD activity. This PLD activation and conidia internalization were inhibited by anti-dectin-1 antibody. Indeed, dectin-1, a β-1,3-glucan receptor, was expressed in A549 cells, and its expression profile was not altered by conidial stimulation. Finally, host cell PLD1 and PLD2 accompanied A. fumigatus conidia during internalization. Our data indicate that host cell PLD activity induced by β-1,3-glucan on the surface of germinated conidia is important for the efficient internalization of A. fumigatus into A549 lung epithelial cells.
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