ArticleThe Journal of biological chemistry2011
Stimulatory effects of calcium on respiration and NAD(P)H synthesis in intact rat heart mitochondria utilizing physiological substrates cannot explain respiratory control in vivo.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 24 citations in OpenAlex.
- A simulation study on the role of mitochondria-sarcoplasmic reticulum CaThe Journal of physiology · 2025Article
- Substrate-dependent differential regulation of mitochondrial bioenergetics in the heart and kidney cortex and outer medulla.Biochimica et biophysica acta. Bioenergetics · 2022Article
- Article
- Quantitative analysis of mitochondrial ATP synthesis.Mathematical biosciences · 2021Article
- Article
- Estrogen maintains mitochondrial content and function in the right ventricle of rats with pulmonary hypertension.Physiological reports · 2017Article
- The effect of respiration buffer composition on mitochondrial metabolism and function.PloS one · 2017Article
- A simulation study on the constancy of cardiac energy metabolites during workload transition.The Journal of physiology · 2016Article
- Improving the physiological realism of experimental models.Interface focus · 2016Review
- Catalytic Coupling of Oxidative Phosphorylation, ATP Demand, and Reactive Oxygen Species Generation.Biophysical journal · 2016Article
- Feedback Regulation and Time Hierarchy of Oxidative Phosphorylation in Cardiac Mitochondria.Biophysical journal · 2016Article
- Open-Loop Control of Oxidative Phosphorylation in Skeletal and Cardiac Muscle Mitochondria by Ca(2.).Biophysical journal · 2016Article
- Regulation of cardiac cellular bioenergetics: mechanisms and consequences.Physiological reports · 2015Article
- What is the function of mitochondrial networks? A theoretical assessment of hypotheses and proposal for future research.BioEssays : news and reviews in molecular, cellular and developmental biology · 2015Review
- Mitochondrial targets for volatile anesthetics against cardiac ischemia-reperfusion injury.Frontiers in physiology · 2014Review
- Extra-matrix Mg2+ limits Ca2+ uptake and modulates Ca2+ uptake-independent respiration and redox state in cardiac isolated mitochondria.Journal of bioenergetics and biomembranes · 2013Article
- Modeling to link regional myocardial work, metabolism and blood flows.Annals of biomedical engineering · 2012Review
- Enhanced charge-independent mitochondrial free Ca(2+) and attenuated ADP-induced NADH oxidation by isoflurane: Implications for cardioprotection.Biochimica et biophysica acta · 2012Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Mitochondrial TCA cycle dehydrogenase enzymes have been shown to be stimulated by Ca(2+) under various substrate and ADP incubation conditions in an attempt to determine and understand the role of Ca(2+) in maintaining energy homeostasis in working hearts. In this study, we tested the hypothesis that, at physiological temperature and 1 mM extramitochondrial free magnesium, Ca(2+) can stimulate the overall mitochondrial NAD(P)H generation flux in rat heart mitochondria utilizing pyruvate and malate as substrates at both subsaturating and saturating concentrations. In both cases, we found that, in the physiological regime of mitochondrial oxygen consumption observed in the intact animal and in the physiological range of cytosolic Ca(2+) concentration averaged per beat, Ca(2+) had no observable stimulatory effect. A modest apparent stimulatory effect (22-27%) was observable at supraphysiological maximal ADP-stimulated respiration at 2.5 mM initial phosphate. The stimulatory effects observed over the physiological Ca(2+) range are not sufficient to make a significant contribution to the control of oxidative phosphorylation in the heart in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.