ArticleThe Journal of biological chemistry2011
Genetic engineering of group 2 sigma factor SigE widely activates expressions of sugar catabolic genes in Synechocystis species PCC 6803.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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45 citing papers in PubMed, 109 citations in OpenAlex.
- Deletion of the gene for a cyanobacterial ribosome-associated protein affects the carbon/nitrogen metabolism.bioRxiv : the preprint server for biology · 2026Article
- Enhanced fumarate production using high-density cultivation of Synechocystis sp. PCC 6803.Microbial cell factories · 2026Article
- Development of phytochemical genomics: From decoding metabolome to functional genomics and biotechnology of plant metabolism.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2025Review
- The regulatory impact of serine/threonine-specific protein phosphorylation among cyanobacteria.Frontiers in plant science · 2025Review
- CyAbrB2 is a nucleoid-associated protein ineLife · 2024Article
- Arginine inhibits the arginine biosynthesis rate-limiting enzyme and leads to the accumulation of intracellular aspartate in Synechocystis sp. PCC 6803.Plant molecular biology · 2024Article
- The genome sequence of Synechocystis sp. PCC 6803 substrain GT-T and its implications for the evolution of PCC 6803 substrains.FEBS open bio · 2023Article
- Regulation of organic acid and hydrogen production by NADH/NADFrontiers in microbiology · 2023Article
- Roles of Close Homologues SigB and SigD in Heat and High Light Acclimation of the CyanobacteriumLife (Basel, Switzerland) · 2022Article
- Combining metabolite doping and metabolic engineering to improve 2-phenylethanol production by engineered cyanobacteria.Frontiers in bioengineering and biotechnology · 2022Article
- Absence of KpsM (Slr0977) Impairs the Secretion of Extracellular Polymeric Substances (EPS) and Impacts Carbon Fluxes inmSphere · 2021Article
- Testing the Potential of Regulatory Sigma Factor Mutants for Wastewater Purification or Bioreactor Run in High Light.Current microbiology · 2020Article
- Article
- Live cyanobacteria produce photocurrent and hydrogen using both the respiratory and photosynthetic systems.Nature communications · 2018Article
- Comparative Targeted Proteomics of the Central Metabolism and Photosystems in SigE Mutant Strains ofMolecules (Basel, Switzerland) · 2018Article
- Comparative Genomics of the Baltic Sea Toxic CyanobacteriaFrontiers in microbiology · 2018Article
- Determination of the Glycogen Content in Cyanobacteria.Journal of visualized experiments : JoVE · 2017Article
- Acclimation to High COPlant physiology · 2017Article
- Editorial: Biotechnology of Microalgae, Based on Molecular Biology and Biochemistry of Eukaryotic Algae and Cyanobacteria.Frontiers in microbiology · 2017Article
- Effects of global transcription factor NtcA on photosynthetic production of ethylene in recombinantBiotechnology for biofuels · 2017Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metabolic engineering of photosynthetic organisms is required for utilization of light energy and for reducing carbon emissions.Control of transcriptional regulators is a powerful approach for changing cellular dynamics, because a set of genes is concomitantly regulated. Here, we show that overexpression of a group 2 σ factor, SigE, enhances the expressions of sugar catabolic genes in the unicellular cyanobacterium, Synechocystis sp. PCC 6803. Transcriptome analysis revealed that genes for the oxidative pentose phosphate pathway and glycogen catabolism are induced by overproduction of SigE. Immunoblotting showed that protein levels of sugar catabolic enzymes, such as glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, glycogen phosphorylase, and isoamylase, are increased. Glycogen levels are reduced in the SigE-overexpressing strain grown under light. Metabolome analysis revealed that metabolite levels of the TCA cycle and acetyl-CoA are significantly altered by SigE overexpression. The SigE-overexpressing strain also exhibited defective growth under mixotrophic or dark conditions. Thus, SigE overexpression changes sugar catabolism at the transcript to phenotype levels, suggesting a σ factor-based engineering method for modifying carbon metabolism in photosynthetic bacteria.
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