ArticleThe Journal of biological chemistry2011
The T cell receptor triggering apparatus is composed of monovalent or monomeric proteins.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 52 citations in OpenAlex.
- 3D localization of retrovirus assembly in the presence of structured background with deep learning.Biophysical journal · 2025Article
- The Structural Biology of T-Cell Antigen Detection at Close Contacts.Immunological reviews · 2025Review
- Pre-T cell receptor localization and trafficking are independent of its signaling.The Journal of cell biology · 2023Article
- Discovering immunoreceptor coupling and organization motifs.Frontiers in immunology · 2023Article
- Surfaces for Study of Receptor Dynamics on T Cells.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Recent advances in understanding TCR signaling: a synaptic perspective.Faculty reviews · 2023Review
- Pre-organized landscape of T cell surface.Frontiers in immunology · 2023Review
- The kinase occupancy of T cell coreceptors reconsidered.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Polyvinyl Chloride Nanoparticles Affect Cell Membrane Integrity by Disturbing the Properties of the Multicomponent Lipid Bilayer inMolecules (Basel, Switzerland) · 2022Article
- Approach to map nanotopography of cell surface receptors.Communications biology · 2022Article
- Rapid statistical discrimination of fluorescence images of T cell receptors on immobilizing surfaces with different coating conditions.Scientific reports · 2021Article
- Rapid formation of human immunodeficiency virus-like particles.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- A biophysical perspective on receptor-mediated virus entry with a focus on HIV.Biochimica et biophysica acta. Biomembranes · 2020Review
- Direct Regulation of the T Cell Antigen Receptor's Activity by Cholesterol.Frontiers in cell and developmental biology · 2020Review
- Coreceptors and TCR Signaling - the Strong and the Weak of It.Frontiers in cell and developmental biology · 2020Review
- Weighted Gene Co-expression Network Analysis of Key Biomarkers Associated With Bronchopulmonary Dysplasia.Frontiers in genetics · 2020Article
- Dual Role of CD4 in Peripheral T Lymphocytes.Frontiers in immunology · 2019Review
- Hydrodynamic trapping measures the interaction between membrane-associated molecules.Scientific reports · 2018Article
- Single-Molecule Light-Sheet Imaging of Suspended T Cells.Biophysical journal · 2018Article
- Monomeric TCRs drive T cell antigen recognition.Nature immunology · 2018Article
Corrections and comments
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Authors and funding
16 authors at 5 institutions in 3 countries.
Funding
Abstract
Understanding the component stoichiometry of the T cell antigen receptor (TCR) triggering apparatus is essential for building realistic models of signal initiation. Recent studies suggesting that the TCR and other signaling-associated proteins are preclustered on resting T cells relied on measurements of the behavior of membrane proteins at interfaces with functionalized glass surfaces. Using fluorescence recovery after photobleaching, we show that, compared with the apical surface, the mobility of TCRs is significantly reduced at Jurkat T cell/glass interfaces, in a signaling-sensitive manner. Using two biophysical approaches that mitigate these effects, bioluminescence resonance energy transfer and two-color coincidence detection microscopy, we show that, within the uncertainty of the methods, the membrane components of the TCR triggering apparatus, i.e. the TCR complex, MHC molecules, CD4/Lck and CD45, are exclusively monovalent or monomeric in human T cell lines, implying that TCR triggering depends only on the kinetics of TCR/pMHC interactions. These analyses also showed that constraining proteins to two dimensions at the cell surface greatly enhances random interactions versus those between the membrane and the cytoplasm. Simulations of TCR-pMHC complex formation based on these findings suggest how unclustered TCR triggering-associated proteins might nevertheless be capable of generating complex signaling outputs via the differential recruitment of cytosolic effectors to the cell membrane.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.