ArticleThe Journal of biological chemistry2011
Evidence for an extended hydrogen bond network in the binding site of the nicotinic receptor: role of the vicinal disulfide of the alpha1 subunit.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Targeted Mutations Activate Allosteric Modulation of α5-Containing Nicotinic Acetylcholine Receptors.ACS chemical neuroscience · 2026Article
- A single main-chain hydrogen bond required to keep GABANature communications · 2025Article
- Dithiol Based on l-Cysteine and Cysteamine as a Disulfide-Reducing Agent.The Journal of organic chemistry · 2022Article
- Interactions of Nereistoxin and Its Analogs with Vertebrate Nicotinic Acetylcholine Receptors and Molluscan ACh Binding Proteins.Marine drugs · 2022Article
- Functional Tolerance to Cysteine Mutations in Human α7 Nicotinic Acetylcholine Receptors.ACS chemical neuroscience · 2020Article
- Broad Analysis of Vicinal Disulfides: Occurrences, Conformations with Cis or with Trans Peptides, and Functional Roles Including Sugar Binding.Journal of molecular biology · 2017Article
- The binding orientation of epibatidine at α7 nACh receptors.Neuropharmacology · 2017Article
- Atom-by-atom engineering of voltage-gated ion channels: magnified insights into function and pharmacology.The Journal of physiology · 2015Review
- Interplay between disulfide bonding and N-glycosylation defines SLC4 Na+-coupled transporter extracellular topography.The Journal of biological chemistry · 2015Article
- Functional evaluation of key interactions evident in the structure of the eukaryotic Cys-loop receptor GluCl.ACS chemical biology · 2014Article
- In vivo incorporation of non-canonical amino acids by using the chemical aminoacylation strategy: a broadly applicable mechanistic tool.Chembiochem : a European journal of chemical biology · 2014Review
- Functional probes of drug-receptor interactions implicated by structural studies: Cys-loop receptors provide a fertile testing ground.Journal of medicinal chemistry · 2014Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The defining feature of the α subunits of the family of nicotinic acetylcholine receptors is a vicinal disulfide between Cys-192 and Cys-193. Although this structure has played a pivotal role in a number of pioneering studies of nicotinic receptors, its functional role in native receptors remains uncertain. Using mutant cycle analysis and unnatural residue mutagenesis, including backbone mutagenesis of the peptide bond of the vicinal disulfide, we have established the presence of a network of hydrogen bonds that extends from that peptide NH, across a β turn to another backbone hydrogen bond, and then across the subunit interface to the side chain of a functionally important Asp residue in the non-α subunit. We propose that the role of the vicinal disulfide is to distort the β turn and thereby properly position a backbone NH for intersubunit hydrogen bonding to the key Asp.
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