ArticleThe Journal of biological chemistry2011
Reactivity of the N-terminal region of fibronectin protein to transglutaminase 2 and factor XIIIA.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 26 citations in OpenAlex.
- Cyclic stretch increases transglutaminase 2-mediated serotonylation of extracellular fibronectin in cultured optic nerve head astrocytes: a pilot study.Matrix biology plus · 2026Article
- Targeting the Primordial Chaperone to Overcome Acquired Drug Resistance in Cancer: TG2-Mediated Autophagy.Biomolecules & therapeutics · 2026Review
- Transglutaminase Activities of Blood Coagulant Factor XIII Are Dependent on the Activation Pathways and on the Substrates.Thrombosis and haemostasis · 2023Article
- Characteristic fragment ions associated with dansyl cadaverine and biotin cadaverine adducts on glutamine.Analytical biochemistry · 2020Article
- Mass spectrometry-based molecular mapping of native FXIIIa cross-links in insoluble fibrin clots.The Journal of biological chemistry · 2019Article
- Serotonylation: Serotonin Signaling and Epigenetics.Frontiers in molecular neuroscience · 2019Review
- Fibronectin Facilitates Enterovirus 71 Infection by Mediating Viral Entry.Journal of virology · 2018Article
- Ranking reactive glutamines in the fibrinogen αC region that are targeted by blood coagulant factor XIII.Blood · 2016Article
- Enhanced B-Cell Receptor Recognition of the Autoantigen Transglutaminase 2 by Efficient Catalytic Self-Multimerization.PloS one · 2015Article
- Activity-regulating structural changes and autoantibody epitopes in transglutaminase 2 assessed by hydrogen/deuterium exchange.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Factor XIII and adipocyte biology.Blood · 2014Article
- Transglutaminase 2-mediated serotonylation in pulmonary hypertension.American journal of physiology. Lung cellular and molecular physiology · 2014Review
- Complex contributions of fibronectin to initiation and maturation of microfibrils.The Biochemical journal · 2013Article
- Transglutaminase 2-specific autoantibodies in celiac disease target clustered, N-terminal epitopes not displayed on the surface of cells.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- Identification of a novel zinc metalloprotease through a global analysis of Clostridium difficile extracellular proteins.PloS one · 2013Article
- Critical role of factor XIII in the initial stages of carbon tetrachloride-induced adult liver remodeling.The American journal of pathology · 2011Article
- Dynamic structure of plasma fibronectin.Critical reviews in biochemistry and molecular biologyReview
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Transglutaminase 2 (TG2) is secreted by a non-classical pathway into the extracellular space, where it has several activities pertinent to fibronectin (FN), including binding to the gelatin-binding domain of FN and acting as an integrin co-receptor. Glutamines in the N-terminal tail of FN are known to be susceptible to transamidation by both TG2 and activated blood coagulation factor XIII (FXIIIa). We used immunoblotting, limited proteolysis, and mass spectrometry to localize glutamines within FN that are subject to TG2-catalyzed incorporation of dansylcadaverine in comparison to residues modified by FXIIIa. Such analysis of plasma FN indicated that Gln-3, Gln-7, and Gln-9 in the N-terminal tail and Gln-246 of the linker between fifth and sixth type I modules ((5)F1 and (6)F1) are transamidated by both enzymes. Only minor incorporation of dansylcadaverine was detected elsewhere. Labeling of C-terminally truncated FN constructs revealed efficient TG2- or FXIIIa-catalyzed dansylcadaverine incorporation into the N-terminal residues of constructs as small as the 29-kDa fragment that includes (1-5)F1 and lacks modules from the adjacent gelatin-binding domain. However, when only (1-3)F1 were present, dansylcadaverine incorporation into the N-terminal residues of FN was lost and instead was in the enzymes, near the active site of TG2 and terminal domains of FXIIIa. Thus, these results demonstrate that FXIIIa and TG2 act similarly on glutamines at either end of (1-5)F1 and transamidation specificity of both enzymes is achieved through interactions with the intact 29K fragment.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.