ArticleThe Journal of biological chemistry2011
Identification of human P2X1 receptor-interacting proteins reveals a role of the cytoskeleton in receptor regulation.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 25 citations in OpenAlex.
- Heat shock protein 90 inhibitors reduce trafficking of ATP-gated P2X1 receptors and human platelet responsiveness.The Journal of biological chemistry · 2012Trial
- Mechanisms of aquaporin-4 vesicular trafficking in mammalian cells.Journal of neurochemistry · 2024Article
- Regulation of P2X1 receptors by modulators of the cAMP effectors PKA and EPAC.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Development of a P2X1-eYFP receptor knock-in mouse to track receptors in real time.Purinergic signalling · 2019Article
- CaThe Journal of biological chemistry · 2019Article
- Organization of ATP-gated P2X1 receptor intracellular termini in apo and desensitized states.The Journal of general physiology · 2019Article
- HSP90 Regulation of P2X7 Receptor Function Requires an Intact Cytoplasmic C-Terminus.Molecular pharmacology · 2016Article
- Role for astroglial α1-adrenoreceptors in gliotransmission and control of synaptic plasticity in the neocortex.Frontiers in cellular neuroscience · 2015Article
- Plasma membrane cholesterol as a regulator of human and rodent P2X7 receptor activation and sensitization.The Journal of biological chemistry · 2014Article
- The trafficking and targeting of P2X receptors.Frontiers in cellular neuroscience · 2013Review
- Mass spectrometry analysis of human P2X1 receptors; insight into phosphorylation, modelling and conformational changes.Journal of neurochemistry · 2012Article
- Molecular and functional properties of P2X receptors--recent progress and persisting challenges.Purinergic signalling · 2012Review
- Imaging P2X4 receptor lateral mobility in microglia: regulation by calcium and p38 MAPK.The Journal of biological chemistry · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
P2X1 receptors are ATP-gated ion channels expressed by smooth muscle and blood cells. Carboxyl-terminally His-FLAG-tagged human P2X1 receptors were stably expressed in HEK293 cells and co-purified with cytoskeletal proteins including actin. Disruption of the actin cytoskeleton with cytochalasin D inhibited P2X1 receptor currents with no effect on the time course of the response or surface expression of the receptor. Stabilization of the cytoskeleton with jasplakinolide had no effect on P2X1 receptor currents but decreased receptor mobility. P2X2 receptor currents were unaffected by cytochalasin, and P2X1/2 receptor chimeras were used to identify the molecular basis of actin sensitivity. These studies showed that the intracellular amino terminus accounts for the inhibitory effects of cytoskeletal disruption similar to that shown for lipid raft/cholesterol sensitivity. Stabilization of the cytoskeleton with jasplakinolide abolished the inhibitory effects of cholesterol depletion on P2X1 receptor currents, suggesting that lipid rafts may regulate the receptor through stabilization of the cytoskeleton. These studies show that the cytoskeleton plays an important role in P2X1 receptor regulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.