ArticleTissue engineering. Part A2012
Initial binding and recellularization of decellularized mouse lung scaffolds with bone marrow-derived mesenchymal stromal cells.
Article in Tissue engineering. Part A, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers, 1 of them a synthesis that pooled it.
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Who cites it
89 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Mesenchymal stem cell therapy for laryngotracheal stenosis: A systematic review of preclinical studies.PloS one · 2017Pooled it
- Bioactive photo-crosslinkable hydrogel based on decellularized dental pulp matrix for functional pulp regeneration.BMC oral health · 2026Article
- Anti-CD31 antibody preconditioning for enhancement of endothelial cell capture and vascularization: a novel strategy for bioengineering lung scaffolds.Journal of biological engineering · 2026Article
- Computer vision to predict cell seeding coverage in re-endothelialized mouse lungs.Scientific reports · 2025Article
- Migration and proliferation drive the emergence of patterns in co-cultures of differentiating vascular progenitor cells.Mathematical biosciences and engineering : MBE · 2024Article
- Orthotopic transplantation of the bioengineered lung using a mouse-scale perfusion-based bioreactor and human primary endothelial cells.Scientific reports · 2024Article
- Mesenchymal cells support the early retention of primary alveolar type 2 cells on acellular mouse lung scaffolds.Regenerative therapy · 2024Article
- Unraveling the Relevance of Tissue-Specific Decellularized Extracellular Matrix Hydrogels for Vocal Fold Regenerative Biomaterials: A Comprehensive Proteomic and In Vitro Study.Advanced nanobiomed research · 2023Article
- Different Decellularization Methods in Bovine Lung Tissue Reveals Distinct Biochemical Composition, Stiffness, and Viscoelasticity in Reconstituted Hydrogels.ACS applied bio materials · 2023Article
- What is the need and why is it time for innovative models for understanding lung repair and regeneration?Frontiers in pharmacology · 2023Review
- Emerging Paradigms in Bioengineering the Lungs.Bioengineering (Basel, Switzerland) · 2022Review
- Cell Inertia: Predicting Cell Distributions in Lung Vasculature to Optimize Re-endothelialization.Frontiers in bioengineering and biotechnology · 2022Article
- Decellularized Extracellular Matrix for Tissue Engineering (Review).Sovremennye tekhnologii v meditsine · 2022Review
- Article
- Recellularization of Native Tissue Derived Acellular Scaffolds with Mesenchymal Stem Cells.Cells · 2021Review
- Lung Extracellular Matrix as a Platform for Lung Organ Bioengineering: Design and Development of Tissue Engineered Lung.Advances in experimental medicine and biology · 2021Review
- Engineering Functional Vasculature in Decellularized Lungs Depends on Comprehensive Endothelial Cell Tropism.Frontiers in bioengineering and biotechnology · 2021Article
- Extracellular matrix remodelling in COPD.European respiratory review : an official journal of the European Respiratory Society · 2020Review
- Extracellular Matrix-Based Biomaterials and Their Influence Upon Cell Behavior.Annals of biomedical engineering · 2020Article
- Porcine Lung-Derived Extracellular Matrix Hydrogel Properties Are Dependent on Pepsin Digestion Time.Tissue engineering. Part C, Methods · 2020Article
29 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recellularization of whole decellularized lung scaffolds provides a novel approach for generating functional lung tissue ex vivo for subsequent clinical transplantation. To explore the potential utility of stem and progenitor cells in this model, we investigated recellularization of decellularized whole mouse lungs after intratracheal inoculation of bone marrow-derived mesenchymal stromal cells (MSCs). The decellularized lungs maintained structural features of native lungs, including intact vasculature, ability to undergo ventilation, and an extracellular matrix (ECM) scaffold consisting primarily of collagens I and IV, laminin, and fibronectin. However, even in the absence of intact cells or nuclei, a number of cell-associated (non-ECM) proteins were detected using mass spectroscopy, western blots, and immunohistochemistry. MSCs initially homed and engrafted to regions enriched in types I and IV collagen, laminin, and fibronectin, and subsequently proliferated and migrated toward regions enriched in types I and IV collagen and laminin but not provisional matrix (fibronectin). MSCs cultured for up to 1 month in either basal MSC medium or in a small airways growth media (SAGM) localized in both parenchymal and airway regions and demonstrated several different morphologies. However, while MSCs cultured in basal medium increased in number, MSCs cultured in SAGM decreased in number over 1 month. Under both media conditions, the MSCs predominantly expressed genes consistent with mesenchymal and osteoblast phenotype. Despite a transient expression of the lung precursor TTF-1, no other airway or alveolar genes or vascular genes were expressed. These studies highlight the power of whole decellularized lung scaffolds to study functional recellularization with MSCs and other cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.