ArticleBioconjugate chemistry2011
Fluorescent DNA nanotags featuring covalently attached intercalating dyes: synthesis, antibody conjugation, and intracellular imaging.
Article in Bioconjugate chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 27 citations in OpenAlex.
- Insights into Two Novel Orthopalladated Chromophores with Antimicrobial Activity againstMolecules (Basel, Switzerland) · 2022Article
- DNA Transformations for Diagnosis and Therapy.Advanced functional materials · 2021Review
- Cyanine Dyes Containing Quinoline Moieties: History, Synthesis, Optical Properties, and Applications.Chemistry (Weinheim an der Bergstrasse, Germany) · 2021Review
- Rapid fluorescence immunoassay of benzo[RSC advances · 2018Article
- Combination probes with intercalating anchors and proximal fluorophores for DNA and RNA detection.Nucleic acids research · 2016Article
- Bright Fluorescent Nanotags from Bottlebrush Polymers with DNA-Tipped Bristles.ACS central science · 2015Article
- Fluorescent probes for nucleic Acid visualization in fixed and live cells.Molecules (Basel, Switzerland) · 2013Review
- Synthesis of quaternary heterocyclic salts.Molecules (Basel, Switzerland) · 2013Article
- Photonic DNA-chromophore nanowire networks: harnessing multiple supramolecular assembly modes.Langmuir : the ACS journal of surfaces and colloids · 2013Article
- Gold nanoparticle-based activatable probe for sensing ultralow levels of prostate-specific antigen.ACS nano · 2013Article
- DNA polyfluorophores for real-time multicolor tracking of dynamic biological systems.Angewandte Chemie (International ed. in English) · 2012Article
- Wavelength Dependence of the Fluorescence Quenching Efficiency of Nearby Dyes by Gold Nanoclusters and Nanoparticles: The Roles of Spectral Overlap and Particle Size.The journal of physical chemistry. C, Nanomaterials and interfaces · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 2 institutions in 2 countries.
Funding
Abstract
We have synthesized fluorescent DNA duplexes featuring multiple thiazole orange (TO) intercalating dyes covalently attached to the DNA via a triazole linkage. The intercalating dyes stabilize the duplex against thermal denaturation and show bright fluorescence in the green region of the spectrum. The emission color can be changed to orange or red by addition of energy-accepting Cy3 or Cy5 dyes attached covalently to the DNA duplex. The dye-modified DNA duplexes were then attached to a secondary antibody for intracellular fluorescence imaging of centrosomes in Drosophila embryos. Bright fluorescent foci were observed at the centrosomes in both the donor (TO) and acceptor (Cy5) channels, because the energy transfer efficiency is moderate. Monitoring the Cy5 emission channel significantly minimized the background signal because of the large shift in emission wavelength allowed by energy transfer.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.