ArticleJournal of tissue engineering and regenerative medicine2012
Phenotypic changes in cultured smooth muscle cells: limitation or opportunity for tissue engineering of hollow organs?
Article in Journal of tissue engineering and regenerative medicine, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Cellulose Plant-Derived Scaffolds as a Tool for Myometrium Modeling.International journal of molecular sciences · 2025Article
- Engineered muscle from micro-channeled PEG scaffold with magnetic FeMaterials today. Bio · 2023Article
- Article
- Myostatin Overexpression and Smad Pathway in Detrusor Derived from Pediatric Patients with End-Stage Lower Urinary Tract Dysfunction.International journal of molecular sciences · 2023Article
- Neurogenic Lower Urinary Tract Dysfunction in Spinal Dysraphism: Morphological and Molecular Evidence in Children.International journal of molecular sciences · 2023Review
- Blood-Vessel-Inspired Hierarchical Trilayer Scaffolds: PCL/Gelatin-Driven Protein Adsorption and Cellular Interaction.Polymers · 2022Article
- Adult stem cell sources for skeletal and smooth muscle tissue engineering.Stem cell research & therapy · 2022Review
- Tissue engineering in reconstructive urology-The current status and critical insights to set future directions-critical review.Frontiers in bioengineering and biotechnology · 2022Review
- Spheroids of Bladder Smooth Muscle Cells for Bladder Tissue Engineering.BioMed research international · 2021Article
- Enzyme-injected method of enzymatic dispersion at low temperature is effective for isolation of smooth muscle cells from human esophagogastric junction.Experimental and therapeutic medicine · 2020Article
- Tissue Engineering in Pediatric Bladder Reconstruction-The Road to Success.Frontiers in pediatrics · 2019Review
- Bioengineered intestinal muscularis complexes with long-term spontaneous and periodic contractions.PloS one · 2018Article
- A Calcium Mediated Mechanism Coordinating Vascular Smooth Muscle Cell Adhesion During KCl Activation.Frontiers in physiology · 2018Article
- Bioengineering the gut: future prospects of regenerative medicine.Nature reviews. Gastroenterology & hepatology · 2016Review
- Stem Cells in Functional Bladder Engineering.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2016Review
- Isolation, expansion and characterization of porcine urinary bladder smooth muscle cells for tissue engineering.Biological procedures online · 2016Article
- Article
- Successful implantation of an engineered tubular neuromuscular tissue composed of human cells and chitosan scaffold.Surgery · 2015Article
- Concurrent generation of functional smooth muscle and endothelial cells via a vascular progenitor.Stem cells translational medicine · 2014Article
- Human amniotic fluid stem cell differentiation along smooth muscle lineage.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2013Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Smooth muscle cells (SMCs) are typically used as a cell source for the reconstruction of hollow organs by conventional tissue engineering techniques. However, the necessity for and advantage of the use of tissue-specific SMCs are unknown. The present study investigated the phenotypic changes that occur following isolation and in vitro expansion of rat SMC populations isolated from three different tissues: the aorta, oesophagus and urinary bladder. rSMCs were isolated by enzymatic dispersion and expanded by conventional cell culture techniques, yielding microscopically homogeneous populations. SMC phenotypes were monitored according to their expression of marker proteins during the first two passages. Two of the three SMC populations (rSMC-a and rSMC-e) showed a marked change in their marker protein profiles during the first two passages, which resulted in a homogeneous phenotype that was neither fully contractile nor fully synthetic. SMCs from the urinary bladder did not show such a shift. Differences between the three rSMC populations were observed with regard to proliferative activity and gene expression patterns, suggesting the retention of some tissue-specific cell characteristics. In summary, phenotypic changes in SMCs occur as a result of conventional cell isolation and expansion techniques, thus questioning the necessity for a tissue-specific cell source for regenerative medicine applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.