ArticleStem cells (Dayton, Ohio)2011
Establishment of human trophoblast progenitor cell lines from the chorion.
Article in Stem cells (Dayton, Ohio), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
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Who cites it
69 citing papers in PubMed.
- An assessment of measurement error when using maternal urinary phthalates as proxies for placental tissue levels in the estimation of the association of prenatal phthalates and infant anogenital distance.Journal of exposure science & environmental epidemiology · 2026Article
- Human cytomegalovirus at the maternal-fetal interface: an overview of pathogenesis, defence, and interventions.Clinical science (London, England : 1979) · 2026Review
- Elevated zygotic let-7f-5p alters developmental trajectories and sex-specific somatic growth.iScience · 2026Article
- The modeling of human implantation and early placentation: achievements and perspectives.Human reproduction update · 2025Review
- Review
- Deciphering the Epigenetic Landscape: Placental Development and Its Role in Pregnancy Outcomes.Stem cell reviews and reports · 2024Review
- Human trophoblast stem cells restrict human cytomegalovirus replication.Journal of virology · 2024Article
- Generation of Human Trophoblast Stem Cell-Dependent Placental In Vitro Models.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Article
- Modelling human placental villous development: designing cultures that reflect anatomy.Cellular and molecular life sciences : CMLS · 2022Review
- Understanding the Immune System in Fetal Protection and Maternal Infections during Pregnancy.Journal of immunology research · 2022Review
- Primary Trophoblast Cultures: Characterization of HLA Profiles and Immune Cell Interactions.Frontiers in immunology · 2022Article
- First trimester mechanisms of gestational sac placental and foetal teratogenicity: a framework for birth cohort studies.Human reproduction update · 2021Review
- Human placental cytotrophoblast epigenome dynamics over gestation and alterations in placental disease.Developmental cell · 2021Article
- Transcriptomic profiling of fetal membranes of mice deficient in biglycan and decorin as a model of preterm birth†.Biology of reproduction · 2021Article
- A toolkit for the application of placental-fetal molecular biomarkers in epidemiologic studies of the fetal origins of chronic disease.Current epidemiology reports · 2021Article
- Unique features and emerging in vitro models of human placental development.Reproductive medicine and biology · 2020Review
- Side-Population Trophoblasts Exhibit the Differentiation Potential of a Trophoblast Stem Cell Population, Persist to Term, and are Reduced in Fetal Growth Restriction.Stem cell reviews and reports · 2020Article
- A Novel Chemically Differentiated Mouse Embryonic Stem Cell-Based Model to Study Liver Stages of Plasmodium berghei.Stem cell reports · 2020Article
- Trisomy 21 is Associated with Caspase-2 Upregulation in Cytotrophoblasts at the Maternal-Fetal Interface.Reproductive sciences (Thousand Oaks, Calif.) · 2020Article
9 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors.
Funding
Abstract
Placental trophoblasts are key determinants of in utero development. Mouse trophoblast (TB) stem cells, which were first derived over a decade ago, are a powerful cell culture model for studying their self-renewal or differentiation. Our attempts to isolate an equivalent population from the trophectoderm of human blastocysts generated colonies that quickly differentiated in vitro. This finding suggested that the human placenta has another progenitor niche. Here, we show that the chorion is one such site. Initially, we immunolocalized pluripotency factors and TB fate determinants in the early gestation placenta, amnion, and chorion. Immunoreactive cells were numerous in the chorion. We isolated these cells and plated them in medium containing fibroblast growth factor which is required for human embryonic stem cell self-renewal, and an inhibitor of activin/nodal signaling. Colonies of polarized cells with a limited lifespan emerged. Trypsin dissociation yielded continuously self-replicating monolayers. Colonies and monolayers formed the two major human TB lineages-multinucleate syncytiotrophoblasts and invasive cytotrophoblasts (CTBs). Transcriptional profiling experiments revealed the factors associated with the self-renewal or differentiation of human chorionic TB progenitor cells (TBPCs). They included imprinted genes, NR2F1/2, HMGA2, and adhesion molecules that were required for TBPC differentiation. Together, the results of these experiments suggested that the chorion is one source of epithelial CTB progenitors. These findings explain why CTBs of fully formed chorionic villi have a modest mitotic index and identify the chorionic mesoderm as a niche for TBPCs that support placental growth.
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