ArticleActa histochemica et cytochemica2011
A novel immunohistochemical staining method allows ultrarapid detection of lymph node micrometastases while conserving antibody.
Article in Acta histochemica et cytochemica, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 34 citations in OpenAlex.
- Rapid immunohistochemistry for intraoperative differentiation between high-grade gliomas and primary central nervous system lymphomas: a multicenter prospective study.Brain tumor pathology · 2026Article
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- Mechanical Microvibration Device Enhancing Immunohistochemistry Efficiency.Engineering in life sciences · 2024Article
- Rapid multiplex immunohistochemistry for characterizing tumor-immune microenvironment.Heliyon · 2024Article
- Multiplex Intraoperative Rapid Immunohistochemistry with Noncontact Antibody Mixing for Distinguishing the Histologic Phenotype of Lung Cancer.Pathobiology : journal of immunopathology, molecular and cellular biology · 2024Observational
- Intraoperative rapid immunohistochemistry with noncontact antibody mixing for undiagnosed pulmonary tumors.Cancer science · 2023Observational
- Stapler-lavage cytology using a new rapid immunocytochemistry for evaluating surgical margin status after pulmonary sublobar resection.General thoracic and cardiovascular surgery · 2022Article
- Novel rapid immunohistochemistry using an alternating current electric field identifies Rac and Cdc42 activation in human colon cancer FFPE tissues.Scientific reports · 2022Article
- Harmonization across programmed death ligand 1 (PD-L1) assays for lung cancer by immunohistochemistry using noncontact alternating current electric field mixing.Thoracic cancer · 2021Article
- Rapid HER2 cytologic fluorescence in situ hybridization for breast cancer using noncontact alternating current electric field mixing.Cancer medicine · 2021Article
- Intraoperative diagnosis of lymph node metastasis during segmentectomy for non-small cell lung cancer by rapid immunohistochemistry using noncontact alternating current electric field mixing.Thoracic cancer · 2020Article
- Magnetically Promoted Rapid Immunofluorescence Staining for Frozen Tissue Sections.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2019Article
- Ultra-fast and automated immunohistofluorescent multistaining using a microfluidic tissue processor.Scientific reports · 2019Article
- Novel method for rapid fluorescence in-situ hybridization of ALK rearrangement using non-contact alternating current electric field mixing.Scientific reports · 2017Article
- CD147 expression correlates with lymph node metastasis in T1-T2 squamous cell carcinoma of the tongue.Oncology letters · 2017Article
- Microfluidics for rapid cytokeratin immunohistochemical staining in frozen sections.Laboratory investigation; a journal of technical methods and pathology · 2017Article
- Novel rapid-immunohistochemistry using an alternating current electric field for intraoperative diagnosis of sentinel lymph nodes in breast cancer.Scientific reports · 2017Article
- Novel method for rapid in-situ hybridization of HER2 using non-contact alternating-current electric-field mixing.Scientific reports · 2016Article
- Novel method for immunofluorescence staining of mammalian eggs using non-contact alternating-current electric-field mixing of microdroplets.Scientific reports · 2015Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed a novel ultrarapid immunohistochemical staining method in which an AC electric field is used to facilitate detection of tumor cells. Frozen sections of non-small cell lung cancer in lymph nodes were fixed in acetone for 2 min, after which they were incubated for 2 min with an anti-pancytokeratin antibody cocktail and then with EnVision(TM) complex under an alternating current (AC) electric field. The sections were then incubated with a chromogen (3,3'diaminobenzidine) for 3 min and counterstained with hematoxylin. This method enabled detection of tumor cells in frozen sections in less than 15 min. In addition, we were able to reduce the amount of antibody used by more than 90% when the sections were incubated under the AC electric field for a longer period. This method could be a useful tool for frozen section diagnosis and research. Furthermore, with this method the cost of immunohistochemical staining can be reduced.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.