ArticleJournal of clinical microbiology2011
Identification of Leishmania spp. by molecular amplification and DNA sequencing analysis of a fragment of rRNA internal transcribed spacer 2.
Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
What it found
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Who cites it
69 citing papers in PubMed.
- Molecular Characterization and Prevalence ofPathogens (Basel, Switzerland) · 2026Article
- Molecular Diagnosis of Leishmaniasis: Development of a qPCR Assay for Genus Detection and Differentiation ofDiagnostics (Basel, Switzerland) · 2026Article
- Integrating molecular tools into leishmaniosis surveillance: evaluation of a commercial qPCR kit in sand fly vectors.Parasites & vectors · 2026Article
- Autochthonous Leishmaniosis in Dogs, Cats, Horses, and Wildlife in the United States of America.Microorganisms · 2026Review
- Autochthonous Leishmaniasis in the United States of America.Microorganisms · 2025Review
- Molecular Assays on Cutaneous Swabs as an Effective, Non-Invasive Diagnostic Technique for Cutaneous Leishmaniasis: Results from a Retrospective Study Conducted in Italy.Tropical medicine and infectious disease · 2025Article
- Molecular diagnosis and epidemiological aspects of cutaneous Leishmaniasis in Aleppo: Current status.Parasite epidemiology and control · 2025Article
- Detection ofJournal of tropical medicine · 2025Article
- Article
- Molecular detection ofParasite epidemiology and control · 2024Article
- Dogs may carry Leishmania tropica and Leishmania major in their blood circulation: a molecular and hematological study.BMC veterinary research · 2024Article
- Unusual Unsatisfactory Treatment in Two Patients with Imported Cutaneous Leishmaniasis.Tropical medicine and infectious disease · 2024Article
- A Diagnostic Algorithm for Detection of Leishmania spp. in Human Fresh and Fixed Tissue Samples.The American journal of tropical medicine and hygiene · 2024Article
- Molecular investigation of Leishmania in sandflies (Diptera: Psychodidae) and rodents (Mammalia: Rodentia) in Nahavand, west of Iran.Parasitology research · 2024Article
- Rapid detection, quantification and speciation ofbioRxiv : the preprint server for biology · 2024Article
- CutaneousmSphere · 2024Review
- CutaneousmedRxiv : the preprint server for health sciences · 2024Article
- Novel detection ofParasitology · 2024Article
- The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2024Article
- Simultaneous targeted amplicon deep sequencing and library preparation for a time and cost-effective universal parasite diagnostic sequencing approach.Parasitology research · 2023Article
9 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Isoenzyme analysis of cultured parasites is the conventional approach for Leishmania species identification. Molecular approaches have the potential to be more sensitive and rapid. We designed PCR generic primers to amplify a segment of the rRNA internal transcribed spacer 2 (ITS2) from multiple Leishmania species. To validate the selected ITS2 fragment, we tested clinical specimens and compared the species results obtained by the molecular approach (PCR followed by DNA sequencing analysis) with those from the parasitologic approach (in vitro culture followed by isoenzyme analysis). Among the 159 patients with clinical specimens positive by both approaches, a total of eight Leishmania species were identified. The species results were concordant for all but two patients: for one patient, the results were Leishmania (Viannia) guyanensis by the molecular approach versus L. (V.) braziliensis by the parasitologic approach; for the other patient, the results were L. (Leishmania) tropica versus L. (L.) major, respectively. ITS2 PCR, followed by sequencing analysis, can be used to detect and discriminate among Leishmania species. The results confirmed our hypothesis that a region of the ITS2 gene can complement the characterization of Leishmania parasites at the species level. The approach we developed can be used as a diagnostic tool in reference laboratories with adequate infrastructure to perform molecular characterization of pathogens.
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