ArticleJournal of clinical microbiology2011
Multiplex real-time PCR melting curve assay to detect drug-resistant mutations of Mycobacterium tuberculosis.
Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 42 citations in OpenAlex.
- A Rapid Genotyping Assay forCurrent developments in nutrition · 2026Article
- Universal primer mediated multiplex asymmetric PCR coupled with hybridization-based electrochemical biosensing for rapid, simultaneous and label-free detection of multiple pathogens.Mikrochimica acta · 2026Article
- A novel one-tube nested fluorescence melting curve analysis for rapid detection of drug-resistant Mycobacterium tuberculosis.BMC microbiology · 2025Article
- An exploratory study on the application of nanopore sequencing for detecting Mycobacterium tuberculosis drug resistance in respiratory specimens.BMC pulmonary medicine · 2025Article
- Clinical Evaluation of a Multiplex PCR Assay for Simultaneous Detection of 18 Respiratory Pathogens in Patients with Acute Respiratory Infections.Pathogens (Basel, Switzerland) · 2022Article
- Identification and Antibiotic-Susceptibility Profiling of Infectious Bacterial Agents: A Review of Current and Future Trends.Biotechnology journal · 2019Review
- Multiplex detection of extensively drug resistant tuberculosis using binary deoxyribozyme sensors.Biosensors & bioelectronics · 2017Article
- Transmission is a Noticeable Cause of Resistance Among Treated Tuberculosis Patients in Shanghai, China.Scientific reports · 2017Article
- Detection of Isoniazid-, Fluoroquinolone-, Amikacin-, and Kanamycin-Resistant Tuberculosis in an Automated, Multiplexed 10-Color Assay Suitable for Point-of-Care Use.Journal of clinical microbiology · 2017Article
- Rapid Detection of Rifampicin- and Isoniazid-ResistantJundishapur journal of microbiology · 2016Article
- Prevalence and transmission of pyrazinamide resistant Mycobacterium tuberculosis in China.Tuberculosis (Edinburgh, Scotland) · 2016Article
- Multi-Fluorescence Real-Time PCR Assay for Detection of RIF and INH Resistance of M. tuberculosis.Frontiers in microbiology · 2016Article
- DMSO Increases Mutation Scanning Detection Sensitivity of High-Resolution Melting in Clinical Samples.Clinical chemistry · 2015Article
- Southern East Asian origin and coexpansion of Mycobacterium tuberculosis Beijing family with Han Chinese.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Locked Nucleic Acid Probe-Based Real-Time PCR Assay for the Rapid Detection of Rifampin-Resistant Mycobacterium tuberculosis.PloS one · 2015Article
- Article
- Triplex real-time PCR melting curve analysis for detecting Mycobacterium tuberculosis mutations associated with resistance to second-line drugs in a single reaction.The Journal of antimicrobial chemotherapy · 2013Article
- Mycobacterium tuberculosis Beijing strains favor transmission but not drug resistance in China.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2012Article
- Rapid, high-throughput detection of rifampin resistance and heteroresistance in Mycobacterium tuberculosis by use of sloppy molecular beacon melting temperature coding.Journal of clinical microbiology · 2012Article
- Molecular methods for pathogen and microbial community detection and characterization: current and potential application in diagnostic microbiology.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2012Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Early diagnosis of drug-resistant Mycobacterium tuberculosis is urgently needed to optimize treatment regimens and to prevent the transmission of resistant strains. Real-time PCR assays have been developed to detect drug resistance rapidly, but none of them have been widely applied due to their complexity, high cost, or requirement for advanced instruments. In this study, we developed a real-time PCR method based on melting curve analysis of dually labeled probes. Six probes targeting the rpoB 81-bp core region, katG315, the inhA promoter, the ahpC promoter, and embB306 were designed and validated with clinical isolates. First, 10 multidrug-resistant (MDR) strains with a wide mutation spectrum were used to analyze the melting temperature (T(m)) deviations of different mutations by single real-time PCR. All mutations can be detected by significant T(m) reductions compared to the wild type. Then, three duplex real-time PCRs, with two probes in each, were developed to detect mutations in 158 MDR isolates. Comparison of the results with the sequencing data showed that all mutations covered by the six probes were detected with 100% sensitivity and 100% specificity. Our method provided a new way to rapidly detect drug-resistant mutations in M. tuberculosis. Compared to other real-time PCR methods, we use fewer probes, which are labeled with the same fluorophore, guaranteeing that this assay can be used for detection in a single fluorescent channel or can be run on single-channel instruments. In conclusion, we have developed a widely applicable real-time PCR assay to detect drug-resistant mutations in M. tuberculosis.
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Registered trials
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