ArticleJournal of clinical microbiology2011
Stability of hepatitis C virus, HIV, and hepatitis B virus nucleic acids in plasma samples after long-term storage at -20°C and -70°C.
Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 47 citations in OpenAlex.
- Use of External Quality Control Material for HIV-1 RNA Testing To Assess the Comparability of Data Generated in Separate Laboratories and the Stability of HIV-1 RNA in Samples after Prolonged Storage.Journal of clinical microbiology · 2018Trial
- The Stability of Never-Thawed Serum Biospecimens Stored in Vapor-Phase Liquid Nitrogen: Select Analytes from the National Health and Nutrition Examination Survey, 1999-2000 and 2005-2006.Biopreservation and biobanking · 2026Article
- Pilot Assessment of RNA Stabilization Methods for Influenza A Virus in Swine Oral Fluids.Pathogens (Basel, Switzerland) · 2026Article
- Environmental and microbial factors shaping SARS-CoV-2 RNA decay in wastewater: insights from batch tests and a lab-scale sewer pipeline simulator.Scientific reports · 2026Article
- Metagenomic sequencing in encephalitis diagnostics: Challenges and opportunities in clinical settings.PloS one · 2026Article
- Exploratory integration of near-infrared spectroscopy with clinical data: a machine learning approach for HCV detection in serum samples.Frontiers in medicine · 2025Article
- Effect of concentration, temperature, and time on the stability of hepatitis C virus nucleic acid in serum.Microbiology spectrum · 2024Article
- Preanalytical variables influencing the interpretation and reporting of biological tests on blood samples of living and deceased donors for human body materials.Cell and tissue banking · 2024Article
- Evaluation of the Aptima HBV Quant Assay Compared to Abbott RealTime M2000 HBV Quant Assay and Procleix Ultrio Plus dHBV Assay in Plasma Samples.Microbiology spectrum · 2022Article
- 903 Protein Saver cards: the best alternative for dried blood spot storage at room temperature for HCV RNA.Scientific reports · 2022Article
- An HIV Diagnostic Testing Algorithm Using the cobas HIV-1/HIV-2 Qualitative Assay for HIV Type Differentiation and Confirmation.Journal of clinical microbiology · 2021Article
- Torque Teno Virus plasma level as novel biomarker of retained immunocompetence in HIV-infected patients.Infection · 2021Article
- Article
- Validation of Spiked Postmortem Blood Samples from Cornea Donors on the Abbott ARCHITECT and m2000 Systems for Viral Infections.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2020Article
- Serological testing for infectious diseases markers of donor specimens from 24 h after death show no significant change in outcomes from other specimens.Cell and tissue banking · 2020Article
- Next generation sequencing of human enterovirus strains from an outbreak of enterovirus A71 shows applicability to outbreak investigations.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2020Article
- Detection and molecular characterisation of a diagnosis escape variant associated with occult hepatitis B virus in Brazil.Memorias do Instituto Oswaldo Cruz · 2017Article
- Molecular evidence of viral DNA in non-small cell lung cancer and non-neoplastic lung.British journal of cancer · 2016Article
- Hepatitis B virus DNA stability in plasma samples under short-term storage at 42°C.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2015Article
- Hepatitis B transmission by cell and tissue allografts: how safe is safe enough?World journal of gastroenterology · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The storage of biological samples may affect detection of viral nucleic acid, yet the stability of viral nucleic acid at standard laboratory storage temperatures (-70°C and -20°C) has not been comprehensively assessed. Deterioration of viral RNA and DNA during storage may affect the detection of viruses, thus leading to an increased likelihood of false-negative results on diagnostic testing. The viral loads of 99 hepatitis C virus (HCV), 41 HIV, and 101 hepatitis B virus (HBV) patient samples were measured before and after storage at -20°C and -70°C for up to 9.1 years using Versant branched DNA assays, Cobas Monitor assays, and/or AmpliPrep/AmpliScreen assays. Clinical samples stored at -20°C for up to 1.2 years and at -70°C for up to 9 years showed a statistically significant difference from baseline with respect to HCV RNA titer, although this difference was not greater than 0.5 log(10) unit. The concentration of HIV RNA in clinical samples stored at -20°C for 2.3 years and at -70°C for up to 9.1 years did not differ significantly from the baseline viral load. HBV DNA-positive clinical samples stored at -20°C for up to 5 years and at -70°C for up to 4 years differed significantly in viral load. In all studies, however, the loss of viral load of HCV, HIV, or HBV in clinical samples tested after storage at -20°C and -70°C for up to 9 years ranged from 0.01 to 0.35 log(10) IU/ml and did not exceed 0.5 log(10), which is the estimated intra-assay variation for molecular tests. Hence, the loss was considered of minimal clinical impact and adequate for the detection of HCV, HIV-1, and HBV nucleic acids using nucleic acid assays for the assessment of the infectious risk of cell, blood, and tissue donors.
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Registered trials
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