ArticleJournal of clinical microbiology2011
Detection of Blastomyces dermatitidis and Histoplasma capsulatum from culture isolates and clinical specimens by use of real-time PCR.
Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers, 1 of them a synthesis that pooled it.
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Who cites it
55 citing papers in PubMed, 1 synthesis or guideline pooled it, 139 citations in OpenAlex.
- Progressive disseminated histoplasmosis: a systematic review on the performance of non-culture-based diagnostic tests.The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious DiseasesPooled it
- Article
- Diagnosing Blastomycosis: A Review of Laboratory Methods and Clinical Utility.Journal of fungi (Basel, Switzerland) · 2025Review
- The Molecular Diagnosis of Invasive Fungal Diseases with a Focus on PCR.Diagnostics (Basel, Switzerland) · 2025Review
- Concurrent Histoplasmosis & Blastomycosis in a Young Woman.Journal of community hospital internal medicine perspectives · 2025Article
- Diagnosis of Human Endemic Mycoses Caused by Thermally Dimorphic Fungi: From Classical to Molecular Methods.Journal of fungi (Basel, Switzerland) · 2024Review
- Molecular Diagnostics for Invasive Fungal Diseases: Current and Future Approaches.Journal of fungi (Basel, Switzerland) · 2024Review
- Validation of a real-time polymerase chain reaction for the detection and quantification of the nucleic acid ofMicrobiology spectrum · 2024Article
- Article
- The Current and Future States of Diagnostic Tests for Histoplasmosis with a Focus on People with HIV and Disseminated Histoplasmosis.Journal of fungi (Basel, Switzerland) · 2023Review
- Article
- Blastomycosis: A Review of Mycological and Clinical Aspects.Journal of fungi (Basel, Switzerland) · 2023Review
- Genitourinary Blastomycosis in a Young Male Patient: A Case Report and Review of Diagnostic Challenges.Case reports in infectious diseases · 2023Article
- Molecular Diagnosis of Endemic Mycoses.Journal of fungi (Basel, Switzerland) · 2022Review
- Human Clinical Isolates of Pathogenic Fungi Are Host to Diverse Mycoviruses.Microbiology spectrum · 2022Article
- Histoplasma capsulatum Complement Fixation and Immunodiffusion Assay Sensitivity in Culture-Confirmed Cases of Histoplasmosis: a 10-Year Retrospective Review (2011 to 2020).Journal of clinical microbiology · 2022Article
- Machine learning to improve the interpretation of intercalating dye-based quantitative PCR results.Scientific reports · 2022Article
- Article
- Answer to December 2021 Photo Quiz.Journal of clinical microbiology · 2021Article
- Disseminated genitourinary histoplasmosis in a patient with AIDS with negative urine antigen testing.BMJ case reports · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Blastomyces dermatitidis and Histoplasma capsulatum are dimorphic fungi that often cause self-limited respiratory infections. However, they may also cause severe disseminated disease, depending on the level of the exposure to the organism and the host immune status. In addition, patients with infections caused by these fungi may have very similar clinical presentations. Although microbiologic culture is a standard method for detecting these pathogens, their recovery may require days to weeks, and the manipulation of cultures presents a significant safety hazard to laboratory personnel. Therefore, the goal of this study was to design a rapid, real-time PCR assay to detect and differentiate B. dermatitidis and H. capsulatum from culture isolates and directly from clinical specimens. Primers and fluorescence resonance energy transfer hybridization probes were designed to target the histidine kinase and glyceraldehyde-3-phosphate dehydrogenase genes of B. dermatitidis and H. capsulatum, respectively. The analytical sensitivity of the assay was determined to be 100 copies/μl for both fungi. From culture isolates, the assay demonstrated 100% specificity and 100% sensitivity for B. dermatitidis and 100% specificity and 94% sensitivity for H. capsulatum. Detection directly from 797 clinical specimens demonstrated specificities and sensitivities of 99% and 86% for B. dermatitidis and 100% and 73% for H. capsulatum compared with the results for culture. This real-time PCR assay provides a rapid method for the detection of B. dermatitidis and H. capsulatum from culture isolates and directly from clinical specimens.
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Registered trials
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