ArticleClinical and vaccine immunology : CVI2011
Comparison of plasmid vaccine immunization schedules using intradermal in vivo electroporation.
Article in Clinical and vaccine immunology : CVI, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- Combined Skin and Muscle DNA Priming Provides Enhanced Humoral Responses to a Human Immunodeficency Virus Type 1 Clade C Envelope Vaccine.Human gene therapy · 2018Trial
- Multifarious immunotherapeutic approaches to cure HIV-1 infection.Human vaccines & immunotherapeutics · 2015Trial
- Different immunogens and prime-boost vaccination strategies affect the efficacy of recombinant candidate vaccines against pathogenic orthopoxviruses.Virology journal · 2024Article
- DNA immunization site determines the level of gene expression and the magnitude, but not the type of the induced immune response.PloS one · 2018Article
- Article
- A plant protein signal sequence improved humoral immune response to HPV prophylactic and therapeutic DNA vaccines.Human vaccines & immunotherapeutics · 2017Article
- Validation of an IFNγ/IL2 FluoroSpot assay for clinical trial monitoring.Journal of translational medicine · 2016Article
- Novel immunological strategies for HIV-1 eradication.Journal of virus eradication · 2015Review
- Article
- Enhanced immunogenicity of an HIV-1 DNA vaccine delivered with electroporation via combined intramuscular and intradermal routes.Journal of virology · 2014Article
- Novel attenuated Chikungunya vaccine candidates elicit protective immunity in C57BL/6 mice.Journal of virology · 2014Article
- Clinical validation of IFNγ/IL-10 and IFNγ/IL-2 FluoroSpot assays for the detection of Tr1 T cells and influenza vaccine monitoring in humans.Human vaccines & immunotherapeutics · 2014Article
- Evaluation of immunogen delivery by DNA immunization using non-invasive bioluminescence imaging.Human vaccines & immunotherapeutics · 2013Article
- Immunization with multiple vaccine modalities induce strong HIV-specific cellular and humoral immune responses.Viral immunology · 2012Article
- Can immunotherapy be useful as a "functional cure" for infection with Human Immunodeficiency Virus-1?Retrovirology · 2012Review
- A combination of intradermal jet-injection and electroporation overcomes in vivo dose restriction of DNA vaccines.Genetic vaccines and therapy · 2012Article
- Rational design of HIV vaccines and microbicides: report of the EUROPRISE annual conference 2011.Journal of translational medicine · 2012Article
- Article
- FluoroSpot Analysis of TLR-Activated Monocytes Reveals Several Distinct Cytokine-Secreting Subpopulations.Scandinavian journal of immunology · 2012Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vivo electroporation (EP) has proven to significantly increase plasmid transfection efficiency and to augment immune responses after immunization with plasmids. In this study, we attempted to establish an immunization protocol using intradermal (i.d.) EP. BALB/c mice were immunized with a plasmid encoding HIV-1 p37Gag, either i.d. with the Derma Vax EP device, intramuscularly (i.m.) without EP, or with combinations of both. A novel FluoroSpot assay was used to evaluate the vaccine-specific cellular immune responses. The study showed that i.d. EP immunizations induced stronger immune responses than i.m. immunizations using a larger amount of DNA and that repeated i.d. EP immunizations induced stronger immune responses than i.m. priming followed by i.d. EP boosting. Two and three i.d. EP immunizations induced immune responses of similar magnitude, and a short interval between immunizations was superior to a longer interval in terms of the magnitude of cellular immune responses. The FluoroSpot assay allowed for the quantification of vaccine-specific cells secreting either gamma interferon (IFN-γ), interleukin-2 (IL-2), or both, and the sensitivity of the assay was confirmed with IFN-γ and IL-2 enzyme-linked immunosorbent spot (ELISpot) assays. The data obtained in this study can aid in the design of vaccine protocols using i.d. EP, and the results emphasize the advantages of the FluoroSpot assay over traditional ELISpot assay and intracellular staining for the detection and quantification of bifunctional vaccine-specific immune responses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.