ArticleProteomics2011
High-sensitivity TFA-free LC-MS for profiling histones.
Article in Proteomics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Challenges in PFAS Postdegradation Analysis: Insights from the PFAS-CTAB Model System.ACS measurement science au · 2025Article
- Hypoxia and hypoxia mimetics differentially modulate histone post-translational modifications.Epigenetics · 2021Article
- Chromatographic efficiency and selectivity in top-down proteomics of histones.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2017Article
- Spinal Muscular Atrophy Biomarker Measurements from Blood Samples in a Clinical Trial of Valproic Acid in Ambulatory Adults.Journal of neuromuscular diseases · 2015Article
- Proteomic challenges: sample preparation techniques for microgram-quantity protein analysis from biological samples.International journal of molecular sciences · 2015Review
- Proteomic profiling identifies specific histone species associated with leukemic and cancer cells.Clinical proteomics · 2015Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
The analysis of proteins by RPLC commonly involves the use of TFA as an ion-pairing agent, even though it forms adducts and suppresses sensitivity. The presence of adducts can complicate protein molecular weight assignment especially when protein isoforms coelute as in the case of histones. To mitigate the complicating effects of TFA adducts in protein LC-MS, we have optimized TFA-free methods for protein separation. Protein standards and histones were used to evaluate TFA-free separations using capillary (0.3 mm id) and nanoscale (0.1 mm id) C(8) columns with the ion-pairing agents, formic acid or acetic acid. The optimized method was then used to examine the applicability of the approach for histone characterization in human cancer cell lines and primary tumor cells from chronic lymphocytic leukemia patients.
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Registered trials
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