ArticleJapanese journal of ophthalmology2011
Diagnosis of ocular toxoplasmosis by two polymerase chain reaction (PCR) examinations: qualitative multiplex and quantitative real-time.
Article in Japanese journal of ophthalmology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Comprehensive polymerase chain reaction assay for detection of pathogenic DNA in lymphoproliferative disorders of the ocular adnexa.Scientific reports · 2016Trial
- A Systematic Review and Meta-Analysis of Diagnostic Test Accuracy for Human Toxoplasmosis: Performance, Populations, and Validation Gaps.Medical devices (Auckland, N.Z.) · 2026Review
- Evaluation of Laboratory Techniques for the Diagnosis of Leptospira-Associated Equine Recurrent Uveitis (ERU) With Focus on the Goldmann-Witmer Coefficient.Veterinary ophthalmology · 2026Article
- An update on immunological and molecular tests and their impact in infectious uveitis.Frontiers in ophthalmology · 2023Review
- Epstein-Barr viral corneal stromal keratitis occurring during rheumatoid arthritis treatment: a case report.BMC ophthalmology · 2022Article
- Practical use of multiplex and broad-range PCR in ophthalmology.Japanese journal of ophthalmology · 2021Review
- Bilateral granulomatous panuveitis in two patients with T-cell type of chronic active Epstein-Barr virus infection.BMC ophthalmology · 2019Article
- Coinfections and differential diagnosis in immunocompetent patients with uveitis of infectious origin.BMC infectious diseases · 2019Article
- Outcome of polymerase chain reaction (PCR) analysis in 100 suspected cases of infectious uveitis.Journal of ophthalmic inflammation and infection · 2018Article
- Successful Treatment of Herpes Simplex Virus (HSV)-1-associated Hemophagocytic Lymphohistiocytosis (HLH) with Acyclovir: A Case Report and Literature Review.Internal medicine (Tokyo, Japan) · 2017Article
- Multiplex polymerase chain reaction for pathogen detection in donor/recipient corneal transplant tissue and donor storage solution.Scientific reports · 2017Article
- Frequency of Toxoplasma gondii in the retina in eye banks in Brazil.Parasitology research · 2017Article
- A new era of uveitis: impact of polymerase chain reaction in intraocular inflammatory diseases.Japanese journal of ophthalmology · 2017Review
- Molecular diagnosis of Toxoplasma gondii infection in Libya.BMC infectious diseases · 2016Article
- Development and validation of new diagnostic criteria for acute retinal necrosis.Japanese journal of ophthalmology · 2015Article
- Seroprevalences of Toxoplasma gondii and Neospora caninum in pet rabbits in Japan.The Journal of veterinary medical science · 2014Article
- Presumed toxoplasmic central retinal artery occlusion and multifocal retinitis with perivascular sheathing.Clinical ophthalmology (Auckland, N.Z.) · 2014Article
- Role of molecular diagnostics in ocular microbiology.Current ophthalmology reports · 2013Article
- The value of routine polymerase chain reaction analysis of intraocular fluid specimens in the diagnosis of infectious posterior uveitis.TheScientificWorldJournal · 2013Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo establish a two-step polymerase chain reaction (PCR) diagnostic system for ocular toxoplasmosis.
methodsA total of 13 ocular fluid samples (11 aqueous humor and 2 vitreous fluid) were collected from 13 patients with clinically suspected ocular toxoplasmosis. Ten ocular samples from other uveitis patients and 20 samples from subjects without ocular inflammation were used as controls. Two polymerase chain reaction (PCR) methods, i.e., qualitative multiplex PCR and quantitative real-time PCR, were used to measure the toxoplasma genome (T. gondii B1 gene).
resultsQualitative multiplex PCR detected T. gondii B1 gene in the ocular fluids of 11 out of 13 patients with clinically suspected ocular toxoplasmosis. In real-time PCR, we detected high copy numbers of T. gondii DNA (5.1 × 10(2)-2.1 × 10(6) copies/mL) in a total of 10 patients (10/13, 77%). Only ocular toxoplasmosis scar lesions were observed in the three real-time PCR-negative patients. PCR assay results for the samples from the two control groups were all negative.
conclusionsThe two-step PCR examination to detect toxoplasma DNA is a useful tool for diagnosing ocular toxoplasmosis.
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