Evidence map›Paper›PMID 21750879›Full record

ArticleAnalytical and bioanalytical chemistry2011

Screening a fragment cocktail library using ultrafiltration.

Sayaka Shibata, Zhongsheng Zhang, Konstantin V Korotkov, Jaclyn Delarosa, Alberto Napuli, Angela M Kelley, Natasha Mueller, Jennifer Ross, Frank H Zucker, Frederick S Buckner and 5 more

Abstract readValidation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical and bioanalytical chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Sayaka ShibataDepartment of Biochemistry, University of Washington, Seattle, WA 98195, USA.
Zhongsheng Zhang
Konstantin V Korotkov
Jaclyn Delarosa
Alberto Napuli
Angela M Kelley
Natasha Mueller
Jennifer Ross
Frank H Zucker
Frederick S Buckner
Ethan A Merritt
Christophe L M J Verlinde
Wesley C Van Voorhis
Wim G J Hol
Erkang Fan

Funding

Structural Genomics of Pathogenic ProtozoaP50GM064655 · NIGMS · UNIVERSITY OF WASHINGTON · PI HOL, WILHELMUS G. J. · 2001 to 2004
$25.3M
Target Selection and Ligand DiscoveryP01AI067921 · NIAID · UNIVERSITY OF WASHINGTON · PI HOL, WILHELMUS G. J. · 2006 to 2008
$6.7M
STRUCTURAL STUDY OF HEAT-LABILE ENTEROTOXINR01AI034501 · NIAID · UNIVERSITY OF WASHINGTON · PI HOL, WILHELMUS G. J. · 1993 to 2013
$5.4M
Structural Biology of Type II Protein Secretion by Bacterial CellsR56AI034501 · NIAID · UNIVERSITY OF WASHINGTON · PI HOL, WILHELMUS G. J. · 2015 to 2015
$490k
NIAID NIH HHS AI34501NIAID NIH HHS P01 AI067921NIAID NIH HHS P01AI067921NIAID NIH HHS R01 AI034501NIAID NIH HHS R56 AI034501NIGMS NIH HHS P50 GM064655NIGMS NIH HHS P50GM64655
6 · The paper itself

Abstract

Ultrafiltration provides a generic method to discover ligands for protein drug targets with millimolar to micromolar K(d), the typical range of fragment-based drug discovery. This method was tailored to a 96-well format, and cocktails of fragment-sized molecules, with molecular masses between 150 and 300 Da, were screened against medical structural genomics target proteins. The validity of the method was confirmed through competitive binding assays in the presence of ligands known to bind the target proteins.

Indexed as

Binding, CompetitiveDrug DiscoveryEscherichia coliLigandsPlasmodium yoeliiProtein BindingProteinsSmall Molecule LibrariesTrypanosoma brucei bruceiUltrafiltrationLigandsProteinsSmall Molecule Libraries

Identifiers

PMID21750879
PMCPMC3166647

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.